Evidence map›Paper›PMID 31087674›Full record

ReviewBioEssays : news and reviews in molecular, cellular and developmental biology2019

Efficient and Innocuous Live-Cell Delivery: Making Membrane Barriers Disappear to Enable Cellular Biochemistry: How Better Cellular Delivery Tools Can Contribute to Precise and Quantitative Cell Biology Assays.

Jean-Philippe Pellois

Abstract readReview
In one paragraph

Review in BioEssays : news and reviews in molecular, cellular and developmental biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

1 author.

Jean-Philippe PelloisDepartment of Biochemistry and Biophysics, Texas A&M University, College Station, Bldg, room 436, 300 Olsen Blvd, TX, 77843-2128, USA.

Funding

Mechanisms and optimization of endosomal escape for delivery applicationsR01GM110137 · NIGMS · TEXAS A&M AGRILIFE RESEARCH · PI PELLOIS, JEAN-PHILIPPE · 2015 to 2022
$2.3M
NIGMS NIH HHS R01 GM110137
6 · The paper itself

Abstract

The confluence of protein engineering techniques and delivery protocols are providing new opportunities in cell biology. In particular, techniques that render the membrane of cells transiently permeable make the introduction of nongenetically encodable macromolecular probes into cells possible. This, in turn, can enable the monitoring of intracellular processes in ways that can be both precise and quantitative, ushering an area that one may envision as cellular biochemistry. Herein, the author reviews pioneering examples of such new cell-based assays, provides evidence that challenges the paradigm that cell penetration is a necessarily damaging and stressful event for cells, and highlights some of the challenges that should be addressed to fully unlock the potential of this nascent field.

Indexed as

Biological AssayCell MembraneCell Membrane PermeabilityCytological TechniquesDrug Delivery SystemsHalf-LifeHumansProtein BindingProtein EngineeringProtein Processing, Post-TranslationalProteolysisUbiquitinUbiquitincell biochemistrycell biologycell delivery

Identifiers

PMID31087674
PMCPMC6563813

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.