ArticlePhysiological reports2019
Optical recording reveals topological distribution of functionally classified colorectal afferent neurons in intact lumbosacral DRG.
Article in Physiological reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 20 citations in OpenAlex.
- Colorectum and embedded networks of nerve fibers present auxetic responses during uniaxial circumferential extension.Acta biomaterialia · 2026Article
- Suppression of visceral nociception by C-fiber transmission block using temporal interference sinusoidal stimulation.Pain reports · 2026Article
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- Methods for quantitative analyses of nerve fiber deformation in the myenteric plexus under loading of mouse distal colon and rectum.Medical engineering & physics · 2025Article
- Multifunctional Nanomesh Enables Cellular-Resolution, Elastic Neuroelectronics.Advanced materials (Deerfield Beach, Fla.) · 2024Article
- Morphological, molecular, and functional characterization of mouse glutamatergic myenteric neurons.American journal of physiology. Gastrointestinal and liver physiology · 2024Article
- Sex differences in zymosan-induced behavioral visceral hypersensitivity and colorectal afferent sensitization.American journal of physiology. Gastrointestinal and liver physiology · 2024Article
- DRG afferents that mediate physiologic and pathologic mechanosensation from the distal colon.Cell · 2023Article
- Gut-innervating nociceptors regulate the intestinal microbiota to promote tissue protection.Cell · 2022Article
- BzATP Activates Satellite Glial Cells and Increases the Excitability of Dorsal Root Ganglia Neurons In Vivo.Cells · 2022Article
- A Brief Review of In Vitro Models for Injury and Regeneration in the Peripheral Nervous System.International journal of molecular sciences · 2022Review
- Challenges and opportunities in translational pain research - An opinion paper of the working group on translational pain research of the European pain federation (EFIC).European journal of pain (London, England) · 2021Article
- Optical clearing reveals TNBS-induced morphological changes of VGLUT2-positive nerve fibers in mouse colorectum.American journal of physiology. Gastrointestinal and liver physiology · 2021Article
- High-Throughput Functional Characterization of Visceral Afferents by Optical Recordings From Thoracolumbar and Lumbosacral Dorsal Root Ganglia.Frontiers in neuroscience · 2021Article
- Intracolonic Mustard Oil Induces Visceral Pain in Mice by TRPA1-Dependent and -Independent Mechanisms: Role of Tissue Injury and P2X Receptors.Frontiers in pharmacology · 2020Article
- Load-bearing function of the colorectal submucosa and its relevance to visceral nociception elicited by mechanical stretch.American journal of physiology. Gastrointestinal and liver physiology · 2019Article
- Optical recording reveals topological distribution of functionally classified colorectal afferent neurons in intact lumbosacral DRG.Physiological reports · 2019Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Neuromodulation as a non-drug alternative for managing visceral pain in irritable bowel syndrome (IBS) may target sensitized afferents of distal colon and rectum (colorectum), especially their somata in the dorsal root ganglion (DRG). Developing selective DRG stimulation to manage visceral pain requires knowledge of the topological distribution of colorectal afferent somata which are sparsely distributed in the DRG. Here, we implemented GCaMP6f to conduct high-throughput optical recordings of colorectal afferent activities in lumbosacral DRG, that is, optical electrophysiology. Using a mouse ex vivo preparation with distal colorectum and L5-S1 DRG in continuity, we recorded 791 colorectal afferents' responses to graded colorectal distension (15, 30, 40, and 60 mmHg) and/or luminal shear flow (20-30 mL/min), then functionally classified them into four mechanosensitive classes, and determined the topological distribution of their somata in the DRG. Of the 791 colorectal afferents, 90.8% were in the L6 DRG, 8.3% in the S1 DRG, and only 0.9% in the L5 DRG. L6 afferents had all four classes: 29% mucosal, 18.4% muscular-mucosal, 34% low-threshold (LT) muscular, and 18.2% high-threshold (HT) muscular afferents. S1 afferents only had three classes: 19.7% mucosal, 34.8% LT muscular, and 45.5% HT muscular afferents. All seven L5 afferents were HT muscular. In L6 DRG, somata of HT muscular afferents were clustered in the caudal region whereas somata of the other classes did not cluster in specific regions. Outcomes of this study can directly inform the design and improvement of next-generation neuromodulation devices that target the DRG to alleviate visceral pain in IBS patients.
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