ArticleJournal of experimental botany2019
Ammonium and nitrate regulate NH4+ uptake activity of Arabidopsis ammonium transporter AtAMT1;3 via phosphorylation at multiple C-terminal sites.
Article in Journal of experimental botany, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28 citing papers in PubMed, 62 citations in OpenAlex.
- Origin and Evolution of Ammonium Transporters (AMTs) in Plants and Systematic Expression Studies inPlants (Basel, Switzerland) · 2026Article
- Article
- Genome-Wide Analysis of Ammonium Transporter Genes in Flowering Chinese Cabbage and Functional Insights into BcAMT1.1 Under Low-Nitrogen Conditions.Plants (Basel, Switzerland) · 2025Article
- Genome-Wide Identification of theGenes · 2025Article
- Synergistic Effects of Nitrogen Application Enhance Drought Resistance inPlants (Basel, Switzerland) · 2025Article
- SALT OVERLY SENSITIVE2 and AMMONIUM TRANSPORTER1;1 contribute to plant salt tolerance by maintaining ammonium uptake.The Plant cell · 2025Article
- Loss of Arabidopsis ACR11 results in altered C/N balance and high sensitivity to nitrogen toxicity.Frontiers in plant science · 2025Article
- Origin and evolution of auxin-mediated acid growth.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Phosphoregulation in the N-terminus of NRT2.1 affects nitrate uptake by controlling the interaction of NRT2.1 with NAR2.1 and kinase HPCAL1 in Arabidopsis.Journal of experimental botany · 2024Article
- Nitrate and ammonium, the yin and yang of nitrogen uptake: a time-course transcriptomic study in rice.Frontiers in plant science · 2024Article
- Article
- The Root-Colonizing EndophyteInternational journal of molecular sciences · 2023Article
- Article
- Ammonium Uptake, Mediated by Ammonium Transporters, Mitigates Manganese Toxicity in Duckweed,Plants (Basel, Switzerland) · 2023Article
- Sustaining nitrogen dynamics: A critical aspect for improving salt tolerance in plants.Frontiers in plant science · 2023Review
- Nitrogen transport and assimilation in tea plant (Frontiers in plant science · 2023Review
- Article
- Abscisic acid influences ammonium transport via regulation of kinase CIPK23 and ammonium transporters.Plant physiology · 2022Article
- The mycorrhiza-specific ammonium transporter ZmAMT3;1 mediates mycorrhiza-dependent nitrogen uptake in maize roots.The Plant cell · 2022Article
- Primary nutrient sensors in plants.iScience · 2022Review
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In plants, nutrient transporters require tight regulation to ensure optimal uptake in complex environments. The activities of many nutrient transporters are post-translationally regulated by reversible phosphorylation, allowing rapid adaptation to variable environmental conditions. Here, we show that the Arabidopsis root epidermis-expressed ammonium transporter AtAMT1;3 was dynamically (de-)phosphorylated at multiple sites in the cytosolic C-terminal region (CTR) responding to ammonium and nitrate signals. Under ammonium resupply rapid phosphorylation of a Thr residue (T464) in the conserved part of the CTR (CTRC) effectively inhibited AtAMT1;3-dependent NH4+ uptake. Moreover, phosphorylation of Thr (T494), one of three phosphorylation sites in the non-conserved part of the CTR (CRTNC), moderately decreased the NH4+ transport activity of AtAMT1;3, as deduced from functional analysis of phospho-mimic mutants in yeast, oocytes, and transgenic Arabidopsis. Double phospho-mutants indicated a role of T494 in fine-tuning the NH4+ transport activity when T464 was non-phosphorylated. Transient dephosphorylation of T494 with nitrate resupply closely paralleled a transient increase in ammonium uptake. These results suggest that T464 phosphorylation at the CTRC acts as a prime switch to prevent excess ammonium influx, while T494 phosphorylation at the CTRNC fine tunes ammonium uptake in response to nitrate. This provides a sophisticated regulatory mechanism for plant ammonium transporters to achieve optimal ammonium uptake in response to various nitrogen forms.
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