ArticleJournal of experimental botany2019
The cold-induced transcription factor bHLH112 promotes artemisinin biosynthesis indirectly via ERF1 in Artemisia annua.
Article in Journal of experimental botany, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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31 citing papers in PubMed, 84 citations in OpenAlex.
- Review
- Review
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- Genome-wide identification and cold stress expression of inducer of CBF expression (ICE) transcription factors in Taraxacum kok-saghyz.Functional & integrative genomics · 2026Article
- Regulatory architecture of high-altitude adaptation in Artemisia: from signal perception to specialized metabolism.Plant cell reports · 2026Review
- Glycyrrhiza, a commonly used medicinal herb: Review of species classification, pharmacology, active ingredient biosynthesis, and synthetic biology.Journal of advanced research · 2025Review
- Transcriptional Reprogramming Deploys a Compartmentalized 'Timebomb' in Catharanthus roseus to Fend Off Chewing Herbivores.Plant, cell & environment · 2025Article
- Engineering scutellarin biosynthesis in Artemisia annua.Plant cell reports · 2025Article
- AaMYB121, a Novel R2-MYB-like Transcription Factor, Regulates Artemisinin Biosynthesis inInternational journal of molecular sciences · 2025Article
- Dynamic transcriptomics unveils parallel transcriptional regulation in artemisinin and phenylpropanoid biosynthesis pathways under cold stress in Artemisia annua.Scientific reports · 2024Article
- Genome-Wide Identification ofPlants (Basel, Switzerland) · 2024Article
- Advanced metabolic engineering strategies for increasing artemisinin yield inHorticulture research · 2024Article
- Transcriptome analysis reveals regulatory mechanism of methyl jasmonate-induced monoterpenoid biosynthesis inFrontiers in plant science · 2024Article
- The Light- and Jasmonic Acid-InducedInternational journal of molecular sciences · 2023Article
- PnMYB4 negatively modulates saponin biosynthesis inHorticulture research · 2023Article
- TcbHLH14 a Jasmonate Associated MYC2-like Transcription Factor Positively Regulates Pyrethrin Biosynthesis inInternational journal of molecular sciences · 2023Article
- Genome-wide identification and characterisation of bHLH transcription factors in Artemisia annua.BMC plant biology · 2023Article
- From Plant to Yeast-Advances in Biosynthesis of Artemisinin.Molecules (Basel, Switzerland) · 2022Review
- Genome wide association study of frost tolerance in wheat.Scientific reports · 2022Article
- Variation in terpenoids in leaves of Artemisia annua grown under different LED spectra resulting in diverse antimalarial activities against Plasmodium falciparum.BMC plant biology · 2022Article
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Basic helix-loop-helix (bHLH) proteins are the second largest family of transcription factors (TFs) involved in developmental and physiological processes in plants. In this study, 205 putative bHLH TF genes were identified in the genome of Artemisia annua and expression of 122 of these was determined from transcriptomes used to construct the genetic map of A. annua. Analysis of gene expression association allowed division of the 122 bHLH TFs into five groups. Group V, containing 15 members, was tightly associated with artemisinin biosynthesis genes. Phylogenetic analysis indicated that two bHLH TFs, AabHLH106 and AabHLH112, were clustered with Arabidopsis ICE proteins. AabHLH112 was induced by low temperature, while AabHLH106 was not. We therefore chose AabHLH112 for further examination. AabHLH112 was highly expressed in glandular secretory trichomes, flower buds, and leaves. Dual-luciferase assays demonstrated that AabHLH112 enhanced the promoter activity of artemisinin biosynthesis genes and AaERF1, an AP2/ERF TF that directly and positively regulates artemisinin biosynthesis genes. Yeast one-hybrid assays indicated that AabHLH112 could bind to the AaERF1 promoter, but not to the promoters of artemisinin biosynthesis genes. Overexpression of AabHLH112 significantly up-regulated the expression levels of AaERF1 and artemisinin biosynthesis genes and consequently promoted artemisinin production.
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