ArticleBiomedical optics express2019
Full volume super-resolution imaging of thick mitotic spindle using 3D AO STED microscope.
Article in Biomedical optics express, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Inverse scattering in biological samples via beam propagation.Science advances · 2026Article
- Improving the scan throughput of polygon scanners.Biomedical optics express · 2024Article
- Impact of a tilted coverslip on two-photon and STED microscopy.Biomedical optics express · 2024Article
- Adaptive optics in super-resolution microscopy.Biophysics reports · 2021Article
- Aberration correction in stimulated emission depletion microscopy to increase imaging depth in living brain tissue.Neurophotonics · 2021Article
- Subcellular three-dimensional imaging deep through multicellular thick samples by structured illumination microscopy and adaptive optics.Nature communications · 2021Article
- A review of the application of machine learning in molecular imaging.Annals of translational medicine · 2021Review
- Article
- Adaptive optics for high-resolution imaging.Nature reviews. Methods primers · 2021Article
- z-STED Imaging and Spectroscopy to Investigate Nanoscale Membrane Structure and Dynamics.Biophysical journal · 2020Article
- Fluorescence imaging with tailored light.Nanophotonics (Berlin, Germany) · 2019Article
- Improved generative adversarial networks using the total gradient loss for the resolution enhancement of fluorescence images.Biomedical optics express · 2019Article
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Authors and funding
3 authors.
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Abstract
Stimulated emission depletion (STED) nanoscopy is one of a suite of modern optical microscopy techniques capable of bypassing the conventional diffraction limit in fluorescent imaging. STED makes use of a spiral phase mask to enable 2D super-resolution imaging whereas to achieve full volumetric 3D super-resolution an additional bottle-beam phase mask must be applied. The resolution achieved in biological samples 10 µm or thicker is limited by aberrations induced mainly by scattering due to refractive index heterogeneity in the sample. These aberrations impact the fidelity of both types of phase mask, and have limited the application of STED to thicker biological systems. Here we apply an automated adaptive optics solution to correct the performance of both STED masks, enhancing robustness and expanding the capabilities of this nanoscopic technique. Corroboration in terms of successful high-quality imaging of the full volume of a 15µm mitotic spindle with resolution of 50nm x 50nm x 150nm achieved in all three dimensions is presented.
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Registered trials
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