ArticlePloS one2019
Metabolic profiling of zebrafish embryo development from blastula period to early larval stages.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 37 citations in OpenAlex.
- Chemical-Specific Metabolic Disruption during Early Zebrafish Development is Modulated by the Microbiome.Environment & health (Washington, D.C.) · 2026Article
- Glucose Co-Treatment Modulates Caffeine-Induced Transcriptomic Alterations During Embryogenesis in Zebrafish (Biology · 2026Article
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- Myotendinous Junction development and repair.Journal of orthopaedic translation · 2026Review
- Multi-omics reveals glycolipid metabolism processes during the early development of largemouth bass (Micropterus salmoides).BMC genomics · 2026Article
- Single-cell RNA sequencing reveals tissue-specific transcriptomic changes induced by perfluorooctanesulfonic acid (PFOS) in larval zebrafish (Danio rerio).Journal of hazardous materials · 2025Article
- Dynamics in zebrafish development define transcriptomic specificity after angiogenesis inhibitor exposure.Archives of toxicology · 2025Article
- Evolution and Spatiotemporal Expression ofJournal of developmental biology · 2024Article
- Effect of High Glucose on Embryological Development of Zebrafish,International journal of molecular sciences · 2024Article
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- Ecotoxicological evaluation of surface waters in Northern Namibia.Environmental monitoring and assessment · 2024Article
- Gluconeogenesis in the extraembryonic yolk syncytial layer of the zebrafish embryo.PNAS nexus · 2024Article
- Glucose uptake as an alternative to oxygen uptake for assessing metabolic rate inCurrent research in physiology · 2022Article
- Article
- Metabolic Regulation of Inflammasome Activity Controls Embryonic Hematopoietic Stem and Progenitor Cell Production.Developmental cell · 2020Article
- A Great Catch for Investigating Inborn Errors of Metabolism-Insights Obtained from Zebrafish.Biomolecules · 2020Review
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The zebrafish embryo is a popular model for drug screening, disease modelling and molecular genetics. In this study, samples were obtained from zebrafish at different developmental stages. The stages that were chosen were 3/4, 4/5, 24, 48, 72 and 96 hours post fertilization (hpf). Each sample included fifty embryos. The samples were analysed using gas chromatography time-of-flight mass spectrometry (GC-TOF-MS). Principle component analysis (PCA) was applied to get an overview of the data and orthogonal projection to latent structure discriminant analysis (OPLS-DA) was utilised to discriminate between the developmental stages. In this way, changes in metabolite profiles during vertebrate development could be identified. Using a GC-TOF-MS metabolomics approach it was found that nucleotides and metabolic fuel (glucose) were elevated at early stages of embryogenesis, whereas at later stages amino acids and intermediates in the Krebs cycle were abundant. This agrees with zebrafish developmental biology, as organs such as the liver and pancreas develop at later stages. Thus, metabolomics of zebrafish embryos offers a unique opportunity to investigate large scale changes in metabolic processes during important developmental stages in vertebrate development. In terms of stability of the metabolic profile and viability of the embryos, it was concluded at 72 hpf was a suitable time point for the use of zebrafish as a model system in numerous scientific applications.
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Registered trials
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