ArticleNature microbiology2019
SEDS-bPBP pairs direct lateral and septal peptidoglycan synthesis in Staphylococcus aureus.
Article in Nature microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
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Who cites it
77 citing papers in PubMed, 118 citations in OpenAlex.
- RodZ acts through MreBCD to activate the elongasome inJournal of bacteriology · 2026Article
- Defining the order of assembly of theJournal of bacteriology · 2026Article
- Mislocalization of PBPs inMicrobiology spectrum · 2026Article
- Orthogonal cell division mediated by PcdA organizes surface virulence factors to drive staphylococcal abscess community formation.Cell reports · 2026Article
- A broadly conserved gram-positive lipoprotein regulates cell elongation.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- FtsW protein-protein interactions visualized in live Staphylococcus aureus cells by FLIM-FRET.Nature communications · 2026Article
- Penicillin-Binding Protein-4 (PBP4) ofMicroorganisms · 2026Review
- Orthogonal cell division organizes surface virulence factors to drive staphylococcal abscess community formation.bioRxiv : the preprint server for biology · 2026Article
- Defining the order of assembly of thebioRxiv : the preprint server for biology · 2026Article
- Molecular dissection of Class A PBP function uncovers novel features of the non-canonical Clostridioides difficile divisome complex.PLoS genetics · 2025Article
- Peptidoglycan polymerase function and regulation.Microbiology and molecular biology reviews : MMBR · 2025Review
- Engineering bacterial cell morphology for the design of robust cell factories.Biochemistry and biophysics reports · 2025Review
- Review
- A type IV pili-mediated mutualism between two co-resident temperate archaeal viruses and their host.Cell reports · 2025Article
- Microanatomy related biocidal activity at cellular resolution and bone reconstruction potential of PEG EGaIn nanocapsules.NPJ biofilms and microbiomes · 2025Article
- Phenotypic plasticity in cell elongation among closely related bacterial species.Nature communications · 2025Article
- Temperature-dependent regulation of bacterial cell division hydrolases by the coordinated action of a regulatory RNA and the ClpXP protease.Cell surface (Amsterdam, Netherlands) · 2025Article
- Molecular dissection of Class A PBP function uncovers novel features of the non-canonicalbioRxiv : the preprint server for biology · 2025Article
- How do spherical bacteria regulate cell division?Biochemical Society transactions · 2025Review
- Control of morphogenesis during theScience advances · 2025Article
17 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Peptidoglycan (PGN) is the major component of the bacterial cell wall, a structure that is essential for the physical integrity and shape of the cell. Bacteria maintain cell shape by directing PGN incorporation to distinct regions of the cell, namely, through the localization of late-stage PGN synthesis proteins. These include two key protein families, SEDS transglycosylases and bPBP transpeptidases, proposed to function in cognate pairs. Rod-shaped bacteria have two SEDS-bPBP pairs, involved in elongation and division. Here, we elucidate why coccoid bacteria, such as Staphylococcus aureus, also possess two SEDS-bPBP pairs. We determined that S. aureus RodA-PBP3 and FtsW-PBP1 probably constitute cognate pairs of interacting proteins. A lack of RodA-PBP3 resulted in more spherical cells due to deficient sidewall PGN synthesis, whereas depletion of FtsW-PBP1 arrested normal septal PGN incorporation. Although PBP1 is an essential protein, a mutant lacking PBP1 transpeptidase activity is viable, showing that this protein has a second function. We propose that the FtsW-PBP1 pair has a role in stabilizing the divisome at midcell. In the absence of these proteins, the divisome appears as multiple rings or arcs that drive lateral PGN incorporation, leading to cell elongation. We conclude that RodA-PBP3 and FtsW-PBP1 mediate sidewall and septal PGN incorporation, respectively, and that their activity must be balanced to maintain coccoid morphology.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.