Evidence map›Paper›PMID 31084605›Full record

ArticleJournal of nanobiotechnology2019

Role of nanoparticle size and sialic acids in the distinct time-evolution profiles of nanoparticle uptake in hematopoietic progenitor cells and monocytes.

Bart Wathiong, Sarah Deville, An Jacobs, Nick Smisdom, Pascal Gervois, Ivo Lambrichts, Marcel Ameloot, Jef Hooyberghs, Inge Nelissen

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of nanobiotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.4field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Review
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Bart WathiongHealth Department, Flemish Institute For Technological Research (VITO), Boeretang 200, 2400, Mol, Belgium.ORCID http://orcid.org/0000-0002-1459-4618
Sarah DevilleHealth Department, Flemish Institute For Technological Research (VITO), Boeretang 200, 2400, Mol, Belgium.
An JacobsHealth Department, Flemish Institute For Technological Research (VITO), Boeretang 200, 2400, Mol, Belgium.
Nick SmisdomBiomedical Research Institute (BIOMED), Hasselt University, Agoralaan Building C, 3590, Diepenbeek, Belgium.
Pascal GervoisBiomedical Research Institute (BIOMED), Hasselt University, Agoralaan Building C, 3590, Diepenbeek, Belgium.
Ivo LambrichtsBiomedical Research Institute (BIOMED), Hasselt University, Agoralaan Building C, 3590, Diepenbeek, Belgium.
Marcel AmelootBiomedical Research Institute (BIOMED), Hasselt University, Agoralaan Building C, 3590, Diepenbeek, Belgium.
Jef HooyberghsHealth Department, Flemish Institute For Technological Research (VITO), Boeretang 200, 2400, Mol, Belgium.
Inge NelissenHealth Department, Flemish Institute For Technological Research (VITO), Boeretang 200, 2400, Mol, Belgium. inge.nelissen@vito.be.ORCID http://orcid.org/0000-0002-1248-3463
Flemish Institute for Technological Research · BEHasselt University · BE

Funding

Fonds Wetenschappelijk Onderzoek 12S6517NVlaamse Instelling voor Technologisch Onderzoek 1406
6 · The paper itself

Abstract

backgroundHuman hematopoietic progenitor cells (HPCs) are important for cell therapy in cancer and tissue regeneration. In vitro studies have shown a transient association of 40 nm polystyrene nanoparticles (PS NPs) with these cells, which is of interest for intelligent design and application of NPs in HPC-based regenerative protocols. In this study, we aimed to investigate the involvement of nanoparticles' size and membrane-attached glycan molecules in the interaction of HPCs with PS NPs, and compared it with monocytes. Human cord blood-derived HPCs and THP-1 cells were exposed to fluorescently labelled, carboxylated PS NPs of 40, 100 and 200 nm. Time-dependent nanoparticle membrane association and/or uptake was observed by measuring fluorescence intensity of exposed cells at short time intervals using flow cytometry. By pretreating the cells with neuraminidase, we studied the possible effect of membrane-associated sialic acids in the interaction with NPs. Confocal microscopy was used to visualize the cell-specific character of the NP association.

resultsConfocal images revealed that the majority of PS NPs was initially observed to be retained at the outer membrane of HPCs, while the same NPs showed immediate internalization by THP-1 monocytic cells. After prolonged exposure up to 4 h, PS NPs were also observed to enter the HPCs' intracellular compartment. Cell-specific time courses of NP association with HPCs and THP-1 cells remained persistent after cells were enzymatically treated with neuraminidase, but significantly increased levels of NP association could be observed, suggesting a role for membrane-associated sialic acids in this process.

conclusionsWe conclude that the terminal membrane-associated sialic acids contribute to the NP retention at the outer cell membrane of HPCs. This retention behavior is a unique characteristic of the HPCs and is independent of NP size.

Indexed as

Antigens, CD34Biological TransportCell LineCell MembraneCell SurvivalDelayed-Action PreparationsEndocytosisHematopoietic Stem CellsHumansMonocytesNanoparticlesParticle SizePolystyrenesSialic AcidsSurface PropertiesAntigens, CD34Delayed-Action PreparationsPolystyrenesSialic AcidsConfocal imagingFlow cytometryHematopoietic progenitor cellsMonocytesNanoparticlesProteoglycans

Identifiers

PMID31084605
PMCPMC6513515
OpenAlexW2944895635

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.