ArticleMolecular microbiology2019
Snf2 controls pulcherriminic acid biosynthesis and antifungal activity of the biocontrol yeast Metschnikowia pulcherrima.
Article in Molecular microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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47 citing papers in PubMed, 93 citations in OpenAlex.
- Pulcherriminic acid as an antibiofilm effector: expandingJournal of bacteriology · 2026Review
- Antagonism-driven upregulation of the pulcherriminic acid biosynthetic pathway in Metschnikowia pulcherrima and Leptosphaeria maculans interaction.World journal of microbiology & biotechnology · 2026Article
- Pulcherrimin and Beyond: The Multifaceted Role ofJournal of fungi (Basel, Switzerland) · 2026Review
- Uncorking wine yeast genomics from grape to glass.FEMS yeast research · 2026Review
- Unlocking the potential of Metschnikowia pulcherrima: a dive into the genomic and safety characterization of four plant-associated strains.Applied microbiology and biotechnology · 2025Article
- Whole genome analysis and biocontrol potential of endophytic Bacillus cereus EMS1 against Fusarium wilt in banana.World journal of microbiology & biotechnology · 2025Article
- Yeast-secreted compounds with antifungal activity-screening, genetic parts, biosynthetic pathways, and regulation.FEMS yeast research · 2025Review
- Pulcherriminic acid biosynthesis and transport: insights from a heterologous system in Saccharomyces cerevisiae.FEMS yeast research · 2025Article
- Profiles of Killer Systems and Volatile Organic Compounds of Rowanberry and Rosehip-Inhabiting Yeasts Substantiate Implications for Biocontrol.Foods (Basel, Switzerland) · 2025Article
- Flavour chemistry and metabolic engineering of microbial synthetic pyrazines: decoding the path to green manufacturing of food aroma components.Current research in food science · 2025Review
- Longitudinal metagenomics reveals continuous restructuring of soil pathobiome under persistentFrontiers in plant science · 2025Article
- Reversible stochastic epigenetic like silencing of the production of pulcherriminic acid in the antimicrobial antagonist Metschnikowia Pulcherrima.Scientific reports · 2024Article
- Two-Stage Screening ofMicroorganisms · 2024Article
- Article
- Isolation of local strains of the yeast Metschnikowia for biocontrol and lipid production purposes.World journal of microbiology & biotechnology · 2024Article
- Chitinous material bioconversion by three new chitinases from the yeast Mestchnikowia pulcherrima.Microbial cell factories · 2024Review
- Heterologous pulcherrimin production in Saccharomyces cerevisiae confers inhibitory activity on Botrytis conidiation.FEMS yeast research · 2024Article
- Predictability of the community-function landscape in wine yeast ecosystems.Molecular systems biology · 2023Article
- Pantothenate Auxotrophy in a Naturally Occurring Biocontrol Yeast.Applied and environmental microbiology · 2023Article
- Pulcherrimin protects Bacillus subtilis against oxidative stress during biofilm development.NPJ biofilms and microbiomes · 2023Article
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Authors and funding
16 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metschnikowia pulcherrima synthesises the pigment pulcherrimin, from cyclodileucine (cyclo(Leu-Leu)) as a precursor, and exhibits strong antifungal activity against notorious plant pathogenic fungi. This yeast therefore has great potential for biocontrol applications against fungal diseases; particularly in the phyllosphere where this species is frequently found. To elucidate the molecular basis of the antifungal activity of M. pulcherrima, we compared a wild-type strain with a spontaneously occurring, pigmentless, weakly antagonistic mutant derivative. Whole genome sequencing of the wild-type and mutant strains identified a point mutation that creates a premature stop codon in the transcriptional regulator gene SNF2 in the mutant. Complementation of the mutant strain with the wild-type SNF2 gene restored pigmentation and recovered the strong antifungal activity. Mass spectrometry (UPLC HR HESI-MS) proved the presence of the pulcherrimin precursors cyclo(Leu-Leu) and pulcherriminic acid and identified new precursor and degradation products of pulcherriminic acid and/or pulcherrimin. All of these compounds were identified in the wild-type and complemented strain, but were undetectable in the pigmentless snf2 mutant strain. These results thus identify Snf2 as a regulator of antifungal activity and pulcherriminic acid biosynthesis in M. pulcherrima and provide a starting point for deciphering the molecular functions underlying the antagonistic activity of this yeast.
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