ArticleSynthetic and systems biotechnology2019
Design and synthesis of synthetic UP elements for modulation of gene expression in
Article in Synthetic and systems biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 13 citations in OpenAlex.
- Development of a Transcriptional Biosensor for Hydrogen Sulfide That Functions under Aerobic and Anaerobic Conditions.ACS synthetic biology · 2025Article
- Engineering ofInternational journal of molecular sciences · 2022Article
- From macro to micro: a combined bioluminescence-fluorescence approach to monitor bacterial localization.Environmental microbiology · 2021Article
- Approaches to genetic tool development for rapid domestication of non-model microorganisms.Biotechnology for biofuels · 2021Review
- Phenotypic Variation and Carbapenem Resistance Potential in OXA-499-ProducingFrontiers in microbiology · 2020Article
- Engineering strong and stress-responsive promoters inSynthetic and systems biotechnology · 2019Article
- Context-dependency of synthetic minimal promoters in driving gene expression: a case study.Microbial biotechnology · 2019Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metabolic engineering requires fine-tuned gene expression for most pathway optimization applications. To develop a suitable suite of promoters, traditional bacterial promoter engineering efforts have focused on modifications to the core region, especially the -10 and -35 regions, of native promoters. Here, we demonstrate an alternate, unexplored route of promoter engineering through randomization of the UP element of the promoter-a region that contacts the alpha subunit carboxy-terminal domain instead of the sigma subunit of the RNA polymerase holoenzyme. Through this work, we identify five novel UP element sequences through library-based searches in
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Registered trials
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