ArticleBioengineered2019
Different construction strategies affected on the physiology of Pichia pastoris strains highly expressed lipase by transcriptional analysis of key genes.
Article in Bioengineered, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Integrating dynamic modeling and continuous cultivation to enhance recombinant β-glucosidase production in Komagataella phaffii.Bioresources and bioprocessing · 2026Article
- Is heterogeneity in large-scale bioreactors a real problem in recombinant protein synthesis by Pichia pastoris?Applied microbiology and biotechnology · 2023Article
- Recombinant expression of hen egg white lysozyme with the assistance of xylanase fusion partner inBioengineered · 2022Article
- Recombinant Protein Production and Purification Using Eukaryotic Cell Factories.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Improved production of recombinantBioengineered · 2020Article
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8 authors.
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Abstract
We demonstrated previously that expression of Rhizomucor miehei lipase (RML) in Pichia pastoris could be significantly increased by addition of gene propeptide, optimized signal peptide codons and manipulation of gene dosage. In this study, effects of various strategies on the protein synthesis and secretion pathways were analyzed. Using nine strains previously constructed, we evaluated cell culture properties, enzymatic activities, and analyzed transcriptional levels of nine genes involved in protein synthesis and secretion pathways by qPCR. We observed that (i) Addition of propeptide decreased lipase folding stress by down-regulated four UPR-related genes. (ii) Signal peptide codons optimization had no effect on host with no change in the nine detected genes. (iii) Folding stress and limited transport capacity produced when rml gene dosage exceed 2. Different limiting factors on lipase expression in strains with different construction strategies were identified. This study provides a theoretical basis for further improving RML by transforming host.
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