ArticleJournal of microbiology (Seoul, Korea)2019
Expression of sexual genes in Aspergillus fumigatus homogeneous culture produced by vegetative mass mating.
Article in Journal of microbiology (Seoul, Korea), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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3 citing papers in PubMed, 3 citations in OpenAlex.
- The Gβ-like ProteinJournal of fungi (Basel, Switzerland) · 2022Article
- The LAMMER Kinase, LkhA, AffectsFrontiers in cellular and infection microbiology · 2021Article
- Global Sexual Fertility in the Opportunistic PathogenJournal of fungi (Basel, Switzerland) · 2020Article
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Authors and funding
2 authors at 1 institution in 1 country.
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Abstract
There are presently no studies on the genes for sexual development of Aspergillus fumigatus in situ using mating culture, primarily because of challenging experimental conditions that require a significantly long period of induction and produce developmentally heterogenous culture, harboring very few sexual organs. In order to overcome these challenges, we developed an efficient and convenient procedure called 'vegetative mass mating (VeM)' for study at a molecular level. The VeM method enabled production of a developmentally homogenous A. fumigatus culture, harboring many sexual organs in a plate within a short period of two weeks. Feasibility of the use of VeM for functional study of genes during A. fumigatus sexual development was evaluated by analyzing the transcription pattern of genes involved in pheromone signal transduction and regulation of sexual development. Here, we present for the first time, an in situ expression pattern of sexual genes during the mating process, induced by the VeM method, which will enable and promote the sexual development study of A. fumigatus at the molecular level.
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