Evidence map›Paper›PMID 31079308›Full record

ArticleMikrochimica acta2019

Fluorometric and colorimetric dual-readout alkaline phosphatase activity assay based on enzymatically induced formation of colored Au@Ag nanoparticles and an inner filter effect.

Chuanxia Chen, Guanglu Zhang, Pengjuan Ni, Yuanyuan Jiang, Yizhong Lu, Zhengliang Lu

Abstract read
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In one paragraph

Article in Mikrochimica acta, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
2.1field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 32 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Chuanxia ChenSchool of Materials Science and Engineering, University of Jinan, Jinan, 250022, China. mse_chencx@ujn.edu.cn.
Guanglu ZhangCollege of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes (Ministry of Education), Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Institute of Molecular and Nano Science, Shandong Normal University, Jinan, 250014, China.
Pengjuan NiSchool of Materials Science and Engineering, University of Jinan, Jinan, 250022, China.
Yuanyuan JiangSchool of Materials Science and Engineering, University of Jinan, Jinan, 250022, China.
Yizhong LuSchool of Materials Science and Engineering, University of Jinan, Jinan, 250022, China. mse_luyz@ujn.edu.cn.ORCID 0000-0002-0914-2780
Zhengliang LuKey Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, China. zhengliang.lu@yahoo.com.
University of Jinan · CNShandong Normal University · CN

Funding

National Natural Science Foundation of China 21705056
6 · The paper itself

Abstract

An ultrasensitive fluorometric and colorimetric dual-mode assay is described for the determination of the activity of alkaline phosphatase (ALP). ALP catalyzes the decomposition of 2-phospho-L-ascorbic acid, and the ascorbic acid thus generated reduces silver ions. In the presence of gold nanoparticles, gold-silver nanoparticles (Au@Ag NPs) are formed. This is accompanied by a color change form pink to deep yellow. The Au@Ag NPs reduce the fluorescence of blue fluorescent graphene quantum dots due to spectral overlap. The changes of absorbance (measured at 410 and 520 nm) and fluorescence (measured at excitation/emission wavelengths of 346/415 nm) correlate well with the ALP activity in the 0.01-6 mU·mL

Indexed as

AdultAlkaline PhosphataseAscorbic AcidColorimetryEnzyme AssaysGoldGraphiteHumansLimit of DetectionMetal NanoparticlesOxidation-ReductionQuantum DotsSilverSpectrometry, FluorescenceAlkaline Phosphataseascorbate-2-phosphateAscorbic AcidGoldGraphiteSilverAscorbic acidFluorescenceGold-silver nanoparticlesGraphene quantum dotsPyrolysisQuencherSpectral overlap

Identifiers

PMID31079308
OpenAlexW2944781712

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.