Evidence map›Paper›PMID 31079269›Full record

ArticleJournal of assisted reproduction and genetics2019

Changes in the metabolic composition of storage solution with prolonged cold ischemia of the uterus.

Antoine Tardieu, P Chazelas, P-A Faye, F Favreau, L Nadal-Desbarats, C Sallée, F Margueritte, C-Y Couquet, P Marquet, C Barin-Le Guellec and 1 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of assisted reproduction and genetics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.7field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Uterus transplantation: state of the art in 2021.Journal of assisted reproduction and genetics · 2021
    Review
  5. Custodiol-N Is Superior to CustodiolInternational journal of molecular sciences · 2020
    Article
  6. Review
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 1 country.

Antoine TardieuINSERM, Inserm Unit U1248, 87000, Limoges, France. antoine.tardieu@hotmail.fr.ORCID http://orcid.org/0000-0003-4786-1580
P ChazelasFaculty of Medicine, EA 6309 "Maintenance Myélinique et Neuropathies Périphériques", University of Limoges, 87000, Limoges, France.
P-A FayeFaculty of Medicine, EA 6309 "Maintenance Myélinique et Neuropathies Périphériques", University of Limoges, 87000, Limoges, France.
F FavreauFaculty of Medicine, EA 6309 "Maintenance Myélinique et Neuropathies Périphériques", University of Limoges, 87000, Limoges, France.
L Nadal-DesbaratsFaculty of Medicine, University of Tours, 37000, Tours, France.
C SalléeDepartment of Gynecologic and Obstetric, Hospital of Limoges, 87000, Limoges, France.
F MargueritteDepartment of Gynecologic and Obstetric, Hospital of Limoges, 87000, Limoges, France.
C-Y CouquetPlatform of Medicine, Imagery and experimental surgery (MICE), Hospital of Limoges, 87000, Limoges, France.
P MarquetINSERM, Inserm Unit U1248, 87000, Limoges, France.
C Barin-Le GuellecINSERM, Inserm Unit U1248, 87000, Limoges, France.
T GauthierINSERM, Inserm Unit U1248, 87000, Limoges, France.
Hôpital Universitaire Dupuytren · FRInserm · FRHôpital Mère-Enfant · FRUniversité de Tours · FR

Funding

Agence de la biomédecine none
6 · The paper itself

Abstract

introductionThe development of uterine transplantation (UTx) from deceased donors requires knowledge of the tolerance of the uterus to prolonged cold ischemia (CI). This can be evaluated through the use of biological parameters to assess degradation of the organ between its procurement and transplantation. The objective of this study was to analyze changes in the metabolic composition of the storage solution in cases of prolonged CI in uteri from ewes.

methodsEighteen uterine auto-transplantations were performed in ewes. CI time was 1 h (T1) or 24 h (T24). Samples of Celsior® were taken when the explanted uterus was flushed (T0) and at the end of CI. A dual approach to metabolic analyses was followed: targeted biochemical analyses targeting several predefined metabolites and non-targeted metabolomics analyses based on nuclear magnetic resonance (NMR).

resultsMetabolic analyses were performed on 16 explanted uteri. Metabolomic profiles differed significantly between T1 and T24 (p = 0.003). Hypoxia-associated degradation of the organ was demonstrated by the significantly higher lactate levels at T24 than at T1 (p < 0.05), accompanied by cell lysis, and significantly higher levels of creatine kinase activity in T24 than in T1 uteri (p < 0.05). Oxidative stress increased over time, with a significantly higher oxidized glutathione/glutathione ratio for T24 than for T1 uteri (p < 0.05).

conclusionThe metabolic results indicate a significant degradation of the uterus during 24 h of CI. Metabolic analysis of the storage solution could be used as a non-invasive tool for evaluating uterine degradation during CI before transplantation.

Indexed as

Transplantation, AutologousAnimalsCold IschemiaFemaleHumansMagnetic Resonance SpectroscopyMetabolomeModels, AnimalOxidative StressSheepTissue DonorsUterusCold ischemiaMetabolic changesUterine transplantationUterus transplantation

Identifiers

PMID31079269
PMCPMC6603114
OpenAlexW2944276095

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.