ArticleBMC veterinary research2019
Simultaneous detection and differentiation of canine parvovirus and feline parvovirus by high resolution melting analysis.
Article in BMC veterinary research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it, 27 citations in OpenAlex.
- High-resolution melting analysis in veterinary medicine: a systematic review of applications and diagnostic accuracy.Tropical animal health and production · 2025Pooled it
- Gut Microbiome Disruption in Shelter Cats with Feline Panleukopenia: Virome Co-Detection and Enteric Dysbiosis.Biology · 2026Review
- Feline panleukopenia virus and canine parvovirus: development of two qPCR assays with high-resolution melting analysis and molecular epidemiology in dogs and cats from Northen Italy in 2017-2023.Frontiers in veterinary science · 2026Article
- RAA-CRISPR/Cas12a-driven two-tube, one-tube and one-tube-LFS for rapid detection of feline parvovirus.Frontiers in veterinary science · 2025Article
- Rapid visual detection and differentiation of canine and feline parvovirus via a one-tube RPA-CRISPR/Cas13a assay.Frontiers in microbiology · 2025Article
- Rapid detection of feline parvovirus using RAA-CRISPR/Cas12a-based lateral flow strip and fluorescence.Frontiers in microbiology · 2025Article
- Tag, you're it!: viral diseases in native otters of south-central Chile due to coexistence with invasive American mink and domestic dogs.Frontiers in veterinary science · 2025Article
- Molecular Investigation of Small Ruminant Abortions Using a 10-Plex HRM-qPCR Technique: A Novel Approach in Routine Diagnostics.Microorganisms · 2024Article
- High-resolution melting analysis for simultaneous detection and discrimination between wild-type and vaccine strains of feline calicivirus.The veterinary quarterly · 2023Article
- Simultaneous detection of feline parvovirus and feline bocavirus using SYBR Green I-based duplex real-time polymerase chain reaction.3 Biotech · 2021Article
- Direct detection of Corynebacterium striatum, Corynebacterium propinquum, and Corynebacterium simulans in sputum samples by high-resolution melt curve analysis.BMC infectious diseases · 2021Article
- Validation of a novel molecular assay to the diagnostic of COVID-19 based on real time PCR with high resolution melting.PloS one · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundCanine parvovirus (CPV) and feline parvovirus (FPV) are causative agents of diarrhea in dogs and cats, which manifests as depression, vomiting, fever, loss of appetite, leucopenia, and diarrhea in young animals. CPV and FPV can single or mixed infect cats and cause disease. To diagnose sick animals effectively, an effective virus diagnostic and genome typing method with high sensitivity and specificity is required.
resultsIn this study, a conserved segment containing one SNP A4408C of parvovirus was used for real-time PCR amplification. Subsequently, data were auto-analyzed and plotted using Applied Biosystems® High Resolution Melt Software v3.1. Results showed that CPV and FPV can be detected simultaneously in a single PCR reaction. No cross-reactions were observed with canine adenovirus, canine coronavirus, and canine distemper virus. The assay had a detection limit of 4.2 genome copies of CPV and FPV. A total of 80 clinical samples were subjected to this assay, as well as to conventional PCR-sequence assay and virus isolation. Results showed that the percentage of agreement of the assay and other methods are high.
conclusionsIn short, we have developed a diagnostic test for the accurate detection and differentiation of CPV and FPV in fecal samples, which is also cost effective.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.