Evidence map›Paper›PMID 31077141›Full record

ArticleBMC infectious diseases2019

Serotype and molecular diversity of nasopharyngeal Streptococcus pneumoniae isolates from children before and after vaccination with the ten-valent pneumococcal conjugate vaccine (PCV10) in Ethiopia.

Wondewosen Tsegaye Sime, Abraham Aseffa, Yimtubezenash Woldeamanuel, Sarah Brovall, Eva Morfeldt, Birgitta Henriques-Normark

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Article in BMC infectious diseases, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
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10citing papers in PubMed, 1 pooled it
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 1 synthesis or guideline pooled it.

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  9. Pathogens (Basel, Switzerland) · 2020
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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Wondewosen Tsegaye SimeArmauer Hansen Research Institute, Jimma Road, 1005, Addis Ababa, Ethiopia. wondewosentsg@gmail.com.ORCID http://orcid.org/0000-0001-7040-579X
Abraham AseffaArmauer Hansen Research Institute, Jimma Road, 1005, Addis Ababa, Ethiopia.
Yimtubezenash WoldeamanuelDepartment of Microbiology, Immunology and Parasitology, Addis Ababa University, College of Medicine and Health Science, 9086, Addis Ababa, Ethiopia.
Sarah BrovallThe Public Health Agency of Sweden, Stockholm, Sweden.
Eva MorfeldtThe Public Health Agency of Sweden, Stockholm, Sweden.
Birgitta Henriques-NormarkThe Public Health Agency of Sweden, Stockholm, Sweden.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundStreptococcus pneumoniae is a major human pathogen, and nasopharyngeal colonization is the first step for transmission and pathogenesis of pneumococcal diseases. Ethiopia introduced the 10-valent pneumococcal conjugate vaccine (PCV10) in October 2011. Here we studied nasopharyngeal carriage rates of pneumococci in children and analyzed the serotype and genetic diversity of pneumococcal isolates before first dose and after completion of the vaccine.

methodA longitudinal study was conducted from February 2013 to November 2016. Totally 789 infants were enrolled at the age of 6 weeks before first dose of PCV10 vaccination, 206 were re-sampled at the age of 9 months, and 201 at 2 years of age after the final dose of PCV10 at the age of 14 weeks. One hundred sixteen children were followed during all the three sampling periods. A total of 422 nasopharyngeal isolates were serotyped using gel diffusion and the Quellung reaction, 325 were typed with pulsed field gel electrophoresis (PFGE), and 12 were selected for multi locus sequence typing (MLST).

resultsPneumococcal carriage rates at the age of 6 weeks, 9 months and 2 years of age were 26.6% (210/789), 56.8% (117/206) and 48.3% (97/201), respectively. Out of 116 children none of them carried the same strain during the three period and the carriage rate at the age of 6 weeks, 9 months and 2 years were 32.7% (38/116), 59.% (69/116) and 49.1% (57/116) respectively. Totally 59 pneumococcal serotypes were identified among 422 isolates. Serotype 6A (5.0%) dominated followed by 34 (4.5%), 10A (4.0%), 11A (4.0%), 19F (3.8%), 15B (3.8%), 23F (3.6%), and 15A (3.6%). The proportion of non-PCV10 serotypes among the isolates recovered at 6 weeks, 9 months and 2 years was 79.4, 88.9 and 89.7% respectively. Molecular typing of 325 isolates collected at 6 weeks and 9 months of age showed a high genetic diversity.

conclusionThis study highlights the presence of very diverse serotypes in Ethiopia where non-vaccine serotypes were predominant. Completion of the PCV10 schedule was associated with an approximately 50% reduction of vaccine-type carriage and increase of non-vaccine types. PCV13 would potentially reduce vaccine-type carriage by further 10%.

Indexed as

Carrier StateChild, PreschoolElectrophoresis, Gel, Pulsed-FieldEthiopiaFemaleGenetic VariationHumansInfantLongitudinal StudiesMaleMultilocus Sequence TypingNasopharynxPneumococcal VaccinesSerogroupStreptococcus pneumoniaeVaccines, Conjugate10-valent pneumococcal conjugate vaccinePneumococcal VaccinesVaccines, ConjugateCarriageEthiopiaInfantsPCVSerotypeStreptococcus pneumoniaeVaccination

Identifiers

PMID31077141
PMCPMC6511162

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