ArticleBMC evolutionary biology2019
Functional diversification of horizontally acquired glycoside hydrolase family 45 (GH45) proteins in Phytophaga beetles.
Article in BMC evolutionary biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 46 citations in OpenAlex.
- Evolutionary dynamics of plant cell wall-degrading enzymes reflects feeding ecology in weevils.Communications biology · 2026Article
- Comparative genomics reveal signatures of ecological specialization in the striped ambrosia beetle Trypodendron lineatum.BMC genomics · 2026Article
- Tetraopes Milkweed Beetle Genomes Elucidate the Adaptive Basis of a Temperate Coevolutionary Radiation.Molecular ecology resources · 2026Article
- Effects of Supplementing Yeast Culture to Prepartum Cows Under Heat Stress on the Jejunal Microbiota and Metabolites of Calves.Animals : an open access journal from MDPI · 2026Article
- Feature Architecture-Aware Ortholog Search With fDOG Reveals the Distribution of Plant Cell Wall-Degrading Enzymes Across Life.Molecular biology and evolution · 2025Article
- Genome-Wide Identification and Expression Profiling of Glycosidases, Lipases, and Proteases from Invasive Asian Palm Weevil,Insects · 2025Article
- Functional Carbohydrate-Active Enzymes Acquired by Horizontal Gene Transfer from Plants in the Whitefly Bemisia tabaci.Genome biology and evolution · 2025Article
- Metabolic enhancement contributed by horizontal gene transfer is essential for dietary specialization in leaf beetles.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Cellulose degradation in Glenea cantor (Fabricius) (Coleoptera: Cerambycidae): functional characterization of GcEGaseZ7 and Cellulase reveals a novel enzymatic activity.Journal of insect science (Online) · 2024Article
- Genomic evidence for the widespread presence of GH45 cellulases among soil invertebrates.Molecular ecology · 2024Article
- Frequent Acquisition of Glycoside Hydrolase Family 32 (GH32) Genes from Bacteria via Horizontal Gene Transfer Drives Adaptation of Invertebrates to Diverse Sources of Food and Living Habitats.International journal of molecular sciences · 2024Article
- Functional Diversification and the Plant Secondary Cell Wall.Journal of molecular evolution · 2023Review
- Towards an understanding of the enzymatic degradation of complex plant mannan structures.World journal of microbiology & biotechnology · 2023Review
- Study of the dual biological impacts of aqueous extracts of normal and gamma-irradiatedJournal of Taibah University Medical Sciences · 2022Article
- Duplication of horizontally acquired GH5_2 enzymes played a central role in the evolution of longhorned beetles.Molecular biology and evolution · 2022Article
- Myriapod genomes reveal ancestral horizontal gene transfer and hormonal gene loss in millipedes.Nature communications · 2022Article
- Expression and characterization of a family 45 glycosyl hydrolase from Fomitopsis pinicola and comparison to Phanerochaete chrysosporium Cel45A.Enzyme and microbial technology · 2022Article
- Insecticide exposure affects intergenerational patterns of DNA methylation in the Colorado potato beetle,Evolutionary applications · 2021Article
- Culturing-Enriched Metabarcoding Analysis of theInsects · 2020Article
- Analyzing the Substrate Specificity of a Class of Long-Horned-Beetle-Derived Xylanases by Using Synthetic Arabinoxylan Oligo- and Polysaccharides.Chembiochem : a European journal of chemical biology · 2020Article
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3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCellulose, a major polysaccharide of the plant cell wall, consists of β-1,4-linked glucose moieties forming a molecular network recalcitrant to enzymatic breakdown. Although cellulose is potentially a rich source of energy, the ability to degrade it is rare in animals and was believed to be present only in cellulolytic microbes. Recently, it has become clear that some animals encode endogenous cellulases belonging to several glycoside hydrolase families (GHs), including GH45. GH45s are distributed patchily among the Metazoa and, in insects, are encoded only by the genomes of Phytophaga beetles. This study aims to understand both the enzymatic functions and the evolutionary history of GH45s in these beetles.
resultsTo this end, we biochemically assessed the enzymatic activities of 37 GH45s derived from five species of Phytophaga beetles and discovered that beetle-derived GH45s degrade three different substrates: amorphous cellulose, xyloglucan and glucomannan. Our phylogenetic and gene structure analyses indicate that at least one gene encoding a putative cellulolytic GH45 was present in the last common ancestor of the Phytophaga, and that GH45 xyloglucanases evolved several times independently in these beetles. The most closely related clade to Phytophaga GH45s was composed of fungal sequences, suggesting this GH family was acquired by horizontal gene transfer from fungi. Besides the insects, other arthropod GH45s do not share a common origin and appear to have emerged at least three times independently.
conclusionThe rise of functional innovation from gene duplication events has been a fundamental process in the evolution of GH45s in Phytophaga beetles. Both, enzymatic activity and ancestral origin suggest that GH45s were likely an essential prerequisite for the adaptation allowing Phytophaga beetles to feed on plants.
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