Evidence map›Paper›PMID 31076994›Full record

ArticleTropical animal health and production2019

Molecular detection and characterization of Clostridium perfringens toxin genes causing necrotic enteritis in broiler chickens.

N Praveen Kumar, N Vinod Kumar, A Karthik

Abstract read
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In one paragraph

Article in Tropical animal health and production, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
2.2field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 24 citations in OpenAlex.

  1. Article
  2. Prevalence and antimicrobial resistance patterns ofOne health (Amsterdam, Netherlands) · 2025
    Article
  3. Article
  4. Article
  5. Article
  6. Phenotypical Identification and Toxinotyping ofVeterinary medicine international · 2023
    Article
  7. Article
  8. Article
  9. Visual Detection ofMicroorganisms · 2020
    Article
  10. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

N Praveen KumarDepartment of Veterinary Microbiology, College of Veterinary Science, SV Veterinary University, Tirupathi, Andhra Pradesh, India. praveenvetty@gmail.com.
N Vinod KumarDepartment of Veterinary Microbiology, College of Veterinary Science, SV Veterinary University, Tirupathi, Andhra Pradesh, India.
A KarthikDepartment of Veterinary Microbiology, College of Veterinary Science, SV Veterinary University, Tirupathi, Andhra Pradesh, India.
Sri Venkateswara Veterinary University · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A total of 464 samples comprising of cloacal swabs from necrotic enteritis suspected live birds (191), intestinal scrapings from dead birds with symptoms of necrotic enteritis (91), and apparently healthy birds (182) were collected from selected districts of AP. The samples were subjected to multiplex PCR for simultaneous detection of α, β, and β2 toxin genes and uniplex PCR for the detection of NetB gene. Multiplex PCR screening of samples reveled α toxin gene positives from (cpa) 248/282 (87.94%) necrotic enteritis suspected and 40/182 (21.97%) apparently healthy samples. Among cpa positives 142/248 (57.25%) and 3/40 (7.5%) were positive for β2 toxin gene in necrotic enteritis suspected and apparently healthy birds respectively, indicating the involvement of C. perfringens type A, with minor pore forming toxin gene cpb2 in causing necrotic enteritis in poultry. None of the sample was positive for β toxin gene. The present research indicates C. perfringens type A along with β2 toxin gene was responsible for causing necrotic enteritis in broiler chickens in some parts of Andhra Pradesh in India. Phylogenetic relationship of amplified cpa and cpb2 amino acids sequences from present C. perfringens isolates were studied. The analysis reveals the sequence identity of cpb2 gene of the present isolates and variations at both nucleotide and amino acid level with the published sequences of cpb2 gene of C. perfringens isolates from different animal species of the USA, Iran, Netherlands, and Japan.

Indexed as

ChickensAnimalsBacterial ToxinsClostridium InfectionsClostridium perfringensDNA, BacterialEnteritisMultiplex Polymerase Chain ReactionPhylogenyPoultry DiseasesBacterial ToxinsDNA, BacterialAndhra PradeshClostridium perfringensMultiplex PCRNecrotic enteritisPhylogenetic analysis

Identifiers

PMID31076994
OpenAlexW2944651647

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.