ArticlePloS one2019
Genotypic antimicrobial resistance assays for use on E. coli isolates and stool specimens.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed.
- Implementation of wastewater-based epidemiology in a Northern California acute care hospital: a pilot study, June-July 2024.The Journal of hospital infection · 2026Article
- Antimicrobial resistance of Escherichia coli in Hungarian wild rats and characterization of a CTX-M-1 type ESBL plasmid.Scientific reports · 2026Article
- Microbiota profiles and antimicrobial resistance genes in sow fecal samples from farms with and without antibiotic use.Animal bioscience · 2025Article
- Detection of antibiotic-resistance genes in bacterial pathogens using a Cas12a/3D DNAzyme colorimetric paper sensor.Fundamental research · 2025Article
- Context-Seq: CRISPR-Cas9 targeted nanopore sequencing for transmission dynamics of antimicrobial resistance.Nature communications · 2025Article
- High throughput screening for human disease associated-pathogens and antimicrobial resistance genes in migratory birds at ten habitat sites in China.BMC microbiology · 2025Article
- Context-Seq: CRISPR-Cas9 Targeted Nanopore Sequencing for Transmission Dynamics of Antimicrobial Resistance.bioRxiv : the preprint server for biology · 2025Article
- Tracking the Prevalence of Antibiotic Resistance inAntibiotics (Basel, Switzerland) · 2024Article
- Impact of Sulfadoxine-Pyrimethamine and Dihydroartemisinin-Piperaquine as Intermittent Preventive Treatment in Pregnancy on Stool Antimicrobial Resistance Gene Abundance.The American journal of tropical medicine and hygiene · 2024Article
- Impact of Soil Fertilization with Pig Slurry on Antibiotic Residues and Resistance Genes: A Longitudinal Study.Antibiotics (Basel, Switzerland) · 2024Article
- Article
- Sanitation in urban areas may limit the spread of antimicrobial resistance via flies.PloS one · 2024Article
- Rapid Detection of Antimicrobial Resistance Genes in Critically Ill Children Using a Custom TaqMan Array Card.Antibiotics (Basel, Switzerland) · 2023Article
- Selection and evaluation of lactic acid bacteria from chicken feces in Thailand as potential probiotics.PeerJ · 2023Article
- Use of Molecular Methods To DetectJournal of clinical microbiology · 2022Article
- Article
- Molecular and phenotypic characterization of avian pathogenic Escherichia coli isolated from commercial broilers and native chickens.Poultry science · 2022Article
- Fecal Microbiota Functional Gene Effects Related to Single-Dose Antibiotic Treatment of Travelers' Diarrhea.Open forum infectious diseases · 2021Article
- First report of urinary tract infection caused by Comamonas kerstersii in a goat.BMC veterinary research · 2021Article
- Antibiotic Resistance in Recreational Waters: State of the Science.International journal of environmental research and public health · 2020Review
Corrections and comments
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Authors and funding
18 authors.
Funding
Abstract
Antimicrobial resistance (AMR) is an emerging public health problem and methods for surveillance are needed. We designed 85 sequence-specific PCR reactions to detect 79 genes or mutations associated with resistance across 10 major antimicrobial classes, with a focus on E. coli. The 85 qPCR assays demonstrated >99.9% concordance with sequencing. We evaluated the correlation between genotypic resistance markers and phenotypic susceptibility results on 239 E. coli isolates. Both sensitivity and specificity exceeded 90% for ampicillin, ceftriaxone, cefepime, imipenem, ciprofloxacin, azithromycin, gentamicin, amikacin, trimethoprim/sulfamethoxazole, tetracycline, and chloramphenicol phenotypic susceptibility results. We then evaluated the assays on direct stool specimens and observed a sensitivity of 97% ± 5 but, as expected, a lower specificity of 75% ± 31 versus the genotype of the E. coli cultured from stool. Finally, the assays were incorporated into a convenient TaqMan Array Card (TAC) format. These assays may be useful for tracking AMR in E. coli isolates or directly in stool for targeted testing of the fecal antibiotic resistome.
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Registered trials
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