ArticlePloS one2019
Environmental factors influence the Haloferax volcanii S-layer protein structure.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- Assembly, architecture and functional roles of microbial surface layers.Nature reviews. Microbiology · 2026Review
- Assessment of methods for evaluating structural stability of cell envelope fragments in hypersaline brines as biosignatures of ancient microbial life.Scientific reports · 2025Article
- The salty tango of brine composition and UV photochemistry effects on Halobacterium salinarum cell envelope biosignature preservation.Communications biology · 2025Article
- DPANN symbiont ofiScience · 2025Article
- Composition and in situ structure of theScience advances · 2024Article
- Cell cycle dependent coordination of surface layer biogenesis in Caulobacter crescentus.Nature communications · 2024Article
- Article
- Isolation and characterization of a main porin from the outer membrane of Salinibacter ruber.Journal of bioenergetics and biomembranes · 2022Article
- The Human Milk Microbiota Produces Potential Therapeutic Biomolecules and Shapes the Intestinal Microbiota of Infants.International journal of molecular sciences · 2022Article
- High-resolution mapping of metal ions reveals principles of surface layer assembly in Caulobacter crescentus cells.Structure (London, England : 1993) · 2022Article
- Complete atomic structure of a native archaeal cell surface.Cell reports · 2021Article
- Comprehensive glycoproteomics shines new light on the complexity and extent of glycosylation in archaea.PLoS biology · 2021Article
- Molecular Logic of Prokaryotic Surface Layer Structures.Trends in microbiology · 2021Review
- Electrochemical Biosensors Based on S-Layer Proteins.Sensors (Basel, Switzerland) · 2020Review
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
S-layers commonly cover archaeal cell envelopes and are composed of proteins that self-assemble into a paracrystalline surface structure. Despite their detection in almost all archaea, there are few reports investigating the structural properties of these proteins, with no reports exploring this topic for halophilic S-layers. The objective of the present study was to investigate the secondary and tertiary organization of the Haloferax volcanii S-layer protein. Such investigations were performed using circular dichroism, fluorescence spectroscopy, dynamic light scattering and transmission electron microscopy. The protein secondary structure is centered on β-sheets and is affected by environmental pH, with higher disorder in more alkaline conditions. The pH can also affect the protein's tertiary structure, with higher tryptophan side-chain exposure to the medium under the same conditions. The concentrations of Na, Mg and Ca ions in the environment also affect the protein structures, with small changes in α-helix and β-sheet content, as well as changes in tryptophan side chain exposure. These changes in turn influence the protein's functional properties, with cell envelope preparations revealing striking differences when in different salt conditions. Thermal denaturation assays revealed that the protein is stable. It has been reported that the S-layer protein N-glycosylation process is affected by external factors and the present study indicates for the first time changes in the protein structure.
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