Evidence map›Paper›PMID 31074891›Full record

ArticleProtein science : a publication of the Protein Society2019

Predicting and validating a model of suppressor of IKKepsilon through biophysical characterization.

Megan L Machek, Halie A Sonnenschein, Sasha-Kaye I Graham, Flowreen Shikwana, Seung-Hwan L Kim, Selena Garcia DuBar, Ian D Minzer, Ryan Wey, Jessica K Bell

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Protein science : a publication of the Protein Society, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Megan L MachekDepartment of Chemistry & Biochemistry, University of San Diego, San Diego, California, 92110.
Halie A SonnenscheinDepartment of Chemistry & Biochemistry, University of San Diego, San Diego, California, 92110.
Sasha-Kaye I GrahamDepartment of Chemistry & Biochemistry, University of San Diego, San Diego, California, 92110.
Flowreen ShikwanaDepartment of Chemistry & Biochemistry, University of San Diego, San Diego, California, 92110.
Seung-Hwan L KimChemistry Department, University of Richmond, Richmond, Virginia, 23137.
Selena Garcia DuBarChemistry Department, University of Richmond, Richmond, Virginia, 23137.
Ian D MinzerDepartment of Chemistry & Biochemistry, University of San Diego, San Diego, California, 92110.
Ryan WeyACS SEED Scholars, University of San Diego, San Diego, California, 92110.
Jessica K BellDepartment of Chemistry & Biochemistry, University of San Diego, San Diego, California, 92110.ORCID 0000-0003-1455-3274
University of San Diego · USUniversity of Richmond · US

Funding

Center for Clinical and Translational ResearchUL1TR000058 · NCATS · VIRGINIA COMMONWEALTH UNIVERSITY · PI MOELLER, FREDERICK GERARD · 2012 to 2015
$12.5M
Functional Characterization of Suppressor of IKKepsilonR21AI107447 · NIAID · VIRGINIA COMMONWEALTH UNIVERSITY · PI BELL, JESSICA K · 2014 to 2015
$370k
NCATS NIH HHS UL1 TR000058NCATS NIH HHS UL1TR000058NIAID NIH HHS AI107447NIAID NIH HHS R21 AI107447
6 · The paper itself

Abstract

Suppressor of IKKepsilon (SIKE) is a 207 residue protein that is implicated in the TLR3-TANK-binding kinase-1-mediated response to viral infection. SIKE's function in this pathway is unknown, but SIKE forms interactions with two distinct cytoskeletal proteins, α-actinin and tubulin, and SIKE knockout reduces cell migration. As structure informs function and in the absence of solved structural homologs, our studies were directed toward creating a structural model of SIKE through biochemical and biophysical characterization to probe and interrogate SIKE function. Circular dichroism revealed a primarily (73%) helical structure of minimal stability (<T

Indexed as

AnimalsCattleGlutamate DehydrogenaseHumansIntracellular Signaling Peptides and ProteinsModels, MolecularProteolysisSerum Albumin, BovineSpectrometry, FluorescenceTryptophanGlutamate DehydrogenaseIntracellular Signaling Peptides and ProteinsSerum Albumin, BovineSIKE1 protein, humanTryptophancircular dichroismcrosslinkingfluorescence polarizationinnate immunitymolecular modelingSIKE

Identifiers

PMID31074891
PMCPMC6635840
OpenAlexW2944474168

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.