ArticleJournal of clinical laboratory analysis2019
A method for improving the efficiency of DNA extraction from clotted blood samples.
Article in Journal of clinical laboratory analysis, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 32 citations in OpenAlex.
- Using clotted, pelleted blood samples for direct molecular detection of Bartonella spp. in small mammal wildlife surveillance studies.BMC research notes · 2024Article
- Thalassemia screening by third-generation sequencing: Pilot study in a Thai population.Obstetric medicine · 2024Article
- Association of a genetic variant in angiopoietin-like 3 with serum HDL-C and risk of cardiovascular disease: A study of the MASHAD cohort over 6 years.Molecular genetics & genomic medicine · 2024Article
- Association between the rs2241883 polymorphism of the fatty acid-binding protein-1 (FABP1) gene and obesity in a population of MASHAD study cohort.Molecular genetics & genomic medicine · 2023Article
- DNA extraction from clotted blood in genotyping quality.BioTechniques · 2023Article
- A Novel NCSTN Mutation in a Three-Generation Chinese Family with Hidradenitis Suppurative.Journal of healthcare engineering · 2022Article
- Association of ANGPTL3 polymorphisms with high-density lipoprotein cholesterol uptake capacity in patients with cardiovascular disease.Journal of clinical laboratory analysis · 2021Article
- Evaluating the Association betweenIranian journal of public health · 2021Article
- Association of PICK1 and BDNF variations with increased risk of methamphetamine dependence among Iranian population: a case-control study.BMC medical genomics · 2021Article
- A method for improving the efficiency of DNA extraction from clotted blood samples.Journal of clinical laboratory analysis · 2019Article
- Two hours method for RNA and DNA co-extraction from blood of coronary artery disease patients: Fast, simple and economical technique.Pakistan journal of medical sciencesArticle
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundThe efficient and rapid extraction of high-quality genomic DNA from clotted blood samples, which normally have a low yield and poor quality, is an important factor in genomic research. The objective of this study was to develop a simple and safe technique for dispersing the blood clots by the ball bearing metal shots. Normally, such clot samples may not have an acceptable yield by conventional DNA extraction methods. Also, in the present study, we have further investigated to improve salting-out DNA extraction methods.
methodsInitially, 500 µL phosphate-buffered saline (PBS) (1×) and two ball bearing metal shots were added to each tube of the clotted blood sample and then were gently rotated in an electric laboratory rotator for 1 hour at room temperature (18-25°C). Genomic DNA was then extracted from samples using a modified salting-out method and a modified QIAamp
resultsThe yield and purity of DNA obtained by these three methods were significantly different (P < 0.001), with a higher yield in the modified salting-out method.
conclusionsOur proposed modified salting-out method is simple and efficient for the isolation of DNA from old blood clot samples. It is both easy to use and is of low cost in routine laboratory tasks.
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