ArticleBiochemistry2019
rEXPAR: An Isothermal Amplification Scheme That Is Robust to Autocatalytic Parasites.
Article in Biochemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Assessment of salivary microRNA by RT-qPCR: Facing challenges in data interpretation for clinical diagnosis.PloS one · 2024Trial
- Robust Sequence Design Space for the Isothermal Exponential Amplification of Short Oligonucleotides.Small (Weinheim an der Bergstrasse, Germany) · 2024Article
- Current Trends in RNA Virus Detection via Nucleic Acid Isothermal Amplification-Based Platforms.Biosensors · 2024Review
- Exponential amplification by redox cross-catalysis and unmasking of doubly protected molecular probes.Chemical science · 2022Article
- Programmed mechano-chemical coupling in reaction-diffusion active matter.Science advances · 2021Article
- A small-molecule chemical interface for molecular programs.Nucleic acids research · 2021Article
- Distance and Microsphere Aggregation-Based DNA Detection in a Paper-Based Microfluidic Device.SLAS technology · 2020Article
- Isothermal digital detection of microRNAs using background-free molecular circuit.Science advances · 2020Article
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Authors and funding
4 authors.
Funding
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Abstract
In the absence of DNA, a solution containing the four deoxynucleotidetriphosphates (dNTPs), a DNA polymerase, and a nicking enzyme generates a self-replicating mixture of DNA species called parasite. Parasites are problematic in template-based isothermal amplification schemes such as EXPAR as well as in related molecular programming approaches, such as the PEN DNA toolbox. Here we show that using a nicking enzyme with only three letters (C, G, T) in the top strand of its recognition site, such as Nb.BssSI, allows us to change the sequence design of EXPAR templates in a way that prevents the formation of parasites when dATP is removed from the solution. This method allows us to make the EXPAR reaction robust to parasite contamination, a common feature in the laboratory, while keeping it compatible with PEN programs, which we demonstrate by engineering a parasite-proof bistable reaction network.
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Registered trials
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