ArticleMolecular biology reports2019
A simple and effective method to obtain high DNA quality and quantity from Cerrado plant species.
Article in Molecular biology reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Efficient DNA Extraction Coupled with Fluorescence Loop-Mediated Isothermal Amplification (LAMP) for On-Site Early Warning Detection of Vibrio parahaemolyticus.Marine biotechnology (New York, N.Y.) · 2026Article
- An innovative optimized protocol for high-quality genomic DNA extraction from recalcitrant Shea tree (Vitellaria paradoxa, C.F. Gaertn) plant and its suitability for downstream applications.Molecular biology reports · 2024Article
- Physical Pretreatments Applied in Three Commercial Kits for the Extraction of High-Quality DNA from Activated Sewage Sludge.International journal of molecular sciences · 2023Article
- A bHLH transcription factor fromMolecular breeding : new strategies in plant improvement · 2021Article
- SILEX: a fast and inexpensive high-quality DNA extraction method suitable for multiple sequencing platforms and recalcitrant plant species.Plant methods · 2020Article
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2 authors.
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Abstract
Despite the importance in conservation and breeding purposes, molecular studies using Cerrado plant species are still rare, mainly because of their high amounts of secondary compounds, impeding DNA extraction to downstream applications, such as PCR amplification. To date, the DNA extraction methods described are sometimes inadequate for these species, expensive, time-intensive and/or use very toxic reagents. Here, we present a simple and effective method, based on SDS and Triton X-100, to obtain high DNA quality and quantity from Cerrado plant species for molecular biological techniques. The DNA obtained by our protocol was free of contaminants and excellent for enzymatic restriction and PCR amplification. The concentration of extracted DNA across all tested species ranged from 156 to 1166 ng µL
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