Evidence map›Paper›PMID 31073914›Full record

ArticleMolecular biology reports2019

Generation of single-chain Fv antibody fragments against Mu-2-related death-inducing gene in Escherichia coli.

Dimuthu Dhammika Wickramanayake, Jun-Ha Choi, Juhyun Shin, Jae-Wook Oh

Abstract read
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In one paragraph

Article in Molecular biology reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 94% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 2 countries.

Dimuthu Dhammika WickramanayakeDepartment of Animal Biotechnology, College of Animal Bioscience and Technology, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Korea.
Jun-Ha ChoiDepartment of Animal Biotechnology, College of Animal Bioscience and Technology, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Korea.
Juhyun ShinDepartment of Animal Biotechnology, College of Animal Bioscience and Technology, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Korea.
Jae-Wook OhDepartment of Animal Biotechnology, College of Animal Bioscience and Technology, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Korea. ohjw@konkuk.ac.kr.ORCID http://orcid.org/0000-0003-2439-0499
Konkuk University · KR

Funding

National Research Foundation of Korea NRF-2016R1D1A1B03935382National Research Foundation of Korea (NRF) NRF-2014R1A1A2056388
6 · The paper itself

Abstract

Mu-2-related death-inducing (MuD) gene is involved in apoptosis in tumor cells. Although we have previously produced mouse monoclonal antibodies (MAbs) that specifically recognize human MuD, the application scope of MuD MAbs was restricted due to their mouse origin. Therefore, we attempted the generation of single-chain variable fragment (scFv) against MuD. The heavy- and light-chain variable region genes from two MuD hybridomas were isolated by PCR and joined by DNA encoding a (Gly

Indexed as

Amino Acid SequenceAnimalsAntibodies, MonoclonalAntibody SpecificityApoptosis Regulatory ProteinsCloning, MolecularEnzyme-Linked Immunosorbent AssayEscherichia coliGene ExpressionGenetic EngineeringHumansHybridomasMiceMice, Inbred BALB CRecombinant ProteinsSingle-Chain AntibodiesAntibodies, MonoclonalAP5M1 protein, humanApoptosis Regulatory ProteinsRecombinant ProteinsSingle-Chain AntibodiesAntibody engineeringApoptosisE. coli expressionMu-2-related death-inducing geneSingle-chain variable fragment antibody

Identifiers

PMID31073914
OpenAlexW2944093375

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.