Evidence map›Paper›PMID 31073284›Full record

ArticleFood & nutrition research2019

Pomegranate peel polyphenols inhibits inflammation in LPS-induced RAW264.7 macrophages via the suppression of TLR4/NF-κB pathway activation.

Lin Du, Jianke Li, Xitong Zhang, Lifang Wang, Weimin Zhang, Mi Yang, Chen Hou

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Food & nutrition research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
72citing papers in PubMed, 1 pooled it
8.7field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

72 citing papers in PubMed, 1 synthesis or guideline pooled it, 131 citations in OpenAlex.

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  16. Therapeutic Potential of Ellagic Acid in Liver Diseases.Molecules (Basel, Switzerland) · 2025
    Review
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  19. A ProprietaryAntioxidants (Basel, Switzerland) · 2025
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12 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Lin DuDepartment of Food Quality and Safety, College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an, China.
Jianke LiDepartment of Food Quality and Safety, College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an, China.
Xitong ZhangDepartment of Food Quality and Safety, College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an, China.
Lifang WangDepartment of Food Quality and Safety, College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an, China.
Weimin ZhangDepartment of Food Quality and Safety, College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an, China.
Mi YangDepartment of Food Quality and Safety, College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an, China.
Chen HouDepartment of Food Quality and Safety, College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an, China.
Shaanxi Normal University · CNHenan University of Science and Technology · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundsInflammatory response mediated by activated immune cells is a vital process in host defense system while responding to various stresses. Our previous studies have indicated that pomegranate peel polyphenols (PPPs) and their main components punicalagin (PC) and ellagic acid (EA) decreased pro-inflammatory cytokines and inflammatory mediators by regulating the mitogen-activated protein kinases (MAPKs) pathway, but whether these tested polyphenols play an important role in NF-κB signaling pathway, another crucial pathway of inflammation, remains unclear.

objectiveIn this study, we analyzed the anti-inflammatory effect of these polyphenols via TLR4-NF-κB pathway in lipopolysaccharide (LPS)-induced RAW264.7 macrophages.

methodsDifferent concentrations of PPPs, PC, and EA were pre-incubated with RAW264.7 macrophages and then stimulated with LPS (1 μg/mL), and the effects of reactive oxygen species and TLR4 were investigated. Moreover, NF-κB p65 nuclear translocation and phosphorylation, and degradation of IκB were measured by Western blot. Furthermore, the influence of pro-inflammatory cytokines was detected by enzyme-linked immunosorbent assay (ELISA).

resultsOur data showed that PPPs, PC, and EA inhibited LPS-induced intracellular ROS production and suppressed the mRNA and protein expression levels of TLR4 in a dose-dependent manner. Moreover, the anti-inflammatory mechanism was involved in blocking LPS-induced phosphorylation, degradation of IκB, and nuclear translocation of p65. Additionally, PPPs and PC exhibited a stronger anti-inflammatory effect than that of EA.

conclusionThe results indicated that PPPs possess potent anti-inflammatory effect, and PC was the main effective component in PPPs, which provided new insights into the utilization of PPPs to prevent inflammation-associated disorders.

Indexed as

anti-inflammationcell signaling pathwayellagic acidnuclear factor-kappa Bpomegranate peel polyphenolspunicalagintoll-like receptors

Identifiers

PMID31073284
PMCPMC6495109
OpenAlexW2941179909

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.