Evidence map›Paper›PMID 31073231›Full record

ArticleScientific reports2019

MinION sequencing technology to characterize unauthorized GM petunia plants circulating on the European Union market.

Marie-Alice Fraiture, Gabriella Ujhelyi, Jaroslava Ovesná, Dirk Van Geel, Sigrid De Keersmaecker, Assia Saltykova, Nina Papazova, Nancy H C Roosens

Abstract read
In one paragraph

Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Marie-Alice FraitureSciensano, Transversal activities in Applied Genomics (TAG), J. Wytsmanstraat 14, 1050, Brussels, Belgium.
Gabriella UjhelyiNational Food Chain Safety Office, Mester utca 81, H-1024, Budapest, Hungary.
Jaroslava OvesnáCrop Research Institute, Department of Molecular Genetics, Drnovská 507, 161 06, Prague, Czech Republic.
Dirk Van GeelSciensano, Transversal activities in Applied Genomics (TAG), J. Wytsmanstraat 14, 1050, Brussels, Belgium.
Sigrid De KeersmaeckerSciensano, Transversal activities in Applied Genomics (TAG), J. Wytsmanstraat 14, 1050, Brussels, Belgium.
Assia SaltykovaSciensano, Transversal activities in Applied Genomics (TAG), J. Wytsmanstraat 14, 1050, Brussels, Belgium.
Nina PapazovaSciensano, Transversal activities in Applied Genomics (TAG), J. Wytsmanstraat 14, 1050, Brussels, Belgium.
Nancy H C RoosensSciensano, Transversal activities in Applied Genomics (TAG), J. Wytsmanstraat 14, 1050, Brussels, Belgium. nancy.roosens@sciensano.be.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In order to characterize unauthorized genetically modified petunia, an integrated strategy has been applied here on several suspected petunia samples from the European market. More precisely, DNA fragments of interest were produced by DNA walking anchored on key targets, earlier detected by real-time PCR screening analysis, to be subsequently sequenced using the MinION platform from Oxford Nanopore Technologies. This way, the presence of genetically modified petunia was demonstrated via the characterization of their transgene flanking regions as well as unnatural associations of elements from their transgenic cassette.

Indexed as

Chromosome WalkingCzech RepublicEuropean UnionHungaryNanoporesPetuniaPlants, Genetically ModifiedReal-Time Polymerase Chain ReactionSequence Analysis, DNA

Identifiers

PMID31073231
PMCPMC6509135

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.