ArticleProceedings of the National Academy of Sciences of the United States of America2019
RNA ligands activate the Machupo virus polymerase and guide promoter usage.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 20 citations in OpenAlex.
- Mutation of a conserved lysine in the RdRp fingers domain broadly attenuates orthobunyaviruses.Communications biology · 2026Article
- Targeting n-myristoyltransferases promotes a pan-Mammarenavirus inhibition through the degradation of the Z matrix protein.PLoS pathogens · 2024Article
- Structure basis for allosteric regulation of lymphocytic choriomeningitis virus polymerase function by Z matrix protein.Protein & cell · 2023Article
- Structural and functional characterization of the Sin Nombre virus L protein.PLoS pathogens · 2023Article
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- Conformational changes in Lassa virus L protein associated with promoter binding and RNA synthesis activity.Nature communications · 2021Article
- Isolation of Reconstructed Functional Ribonucleoprotein Complexes of Machupo Virus.Journal of virology · 2021Article
- Structural insights into RNA polymerases of negative-sense RNA viruses.Nature reviews. Microbiology · 2021Review
- Complete genome sequence of Tacaribe virus.Archives of virology · 2020Article
- Structural and functional characterization of the severe fever with thrombocytopenia syndrome virus L protein.Nucleic acids research · 2020Article
- Independent inhibition of the polymerase and deubiquitinase activities of the Crimean-Congo Hemorrhagic Fever Virus full-length L-protein.PLoS neglected tropical diseases · 2020Article
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Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Segmented negative-sense (SNS) RNA viruses initiate infection by delivering into cells a suite of genomic RNA segments, each sheathed by the viral nucleocapsid protein and bound by the RNA-dependent RNA-polymerase (RdRP). For the orthomyxovirus influenza and the bunyavirus La Crosse, the 5' end of the genomic RNA binds as a hook-like structure proximal to the active site of the RdRP. Using an in vitro assay for the RNA-dependent RNA-polymerase (RdRP) of the arenavirus Machupo (MACV), we demonstrate that the 5' genomic and antigenomic RNAs of both small and large genome segments stimulate activity in a promoter-specific manner. Functional probing of the activating RNAs identifies intramolecular base-pairing between positions +1 and +7 and a pseudotemplated 5' terminal guanine residue as key for activation. Binding of structured 5' RNAs is a conserved feature of all SNS RNA virus polymerases, implying that promoter-specific RdRP activation extends beyond the arenaviruses. The 5' RNAs and the RNA binding pocket itself represent targets for therapeutic intervention.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.