ArticleEBioMedicine2019
Co-culture of Methanobrevibacter smithii with enterobacteria during urinary infection.
Article in EBioMedicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed, 49 citations in OpenAlex.
- Exploring alterations in the gut resistome in medically treated inflammatory bowel disease patients.BMC microbiology · 2026Article
- Methanobrevibacter smithii activates immune microenvironment of intestinum tenue in a mouse model.Archives of microbiology · 2026Article
- Methanobrevibacter smithii strain U29 whole genome sequence delineates M. smithii intermediate cell variants.BMC research notes · 2025Article
- Metagenomic Comparison of Gut Microbes ofAnimals : an open access journal from MDPI · 2025Article
- Review
- Dysbiosis in Human Urinary Microbiota May Differentiate Patients with a Bladder Cancer.International journal of molecular sciences · 2024Article
- Archaea in the Human Microbiome and Potential Effects on Human Infectious Disease.Emerging infectious diseases · 2024Review
- The discovery ofiScience · 2024Article
- Human associated Archaea: a neglected microbiome worth investigating.World journal of microbiology & biotechnology · 2024Review
- The Archaeome's Role in Colorectal Cancer: Unveiling the DPANN Group and Investigating Archaeal Functional Signatures.Microorganisms · 2023Article
- In-depth metataxonomic investigation reveals low richness, high intervariability, and diverse phylotype candidates of archaea in the human urogenital tract.Scientific reports · 2023Article
- Microbiome and Human Health: Current Understanding, Engineering, and Enabling Technologies.Chemical reviews · 2023Review
- The evolving role of methanogenic archaea in mammalian microbiomes.Frontiers in microbiology · 2023Review
- Dysbiosis of the Human Urinary Microbiome and its Association to Diseases Affecting the Urinary System.Indian journal of microbiology · 2022Review
- Bacteria and Methanogens in the Human Microbiome: a Review of Syntrophic Interactions.Microbial ecology · 2022Review
- Determination of the Optimal Bacterial DNA Extraction Method to Explore the Urinary Microbiota.International journal of molecular sciences · 2022Article
- MeconialCurrent research in microbial sciences · 2021Article
- Article
- Preoperative urine nitrite versus urine culture for predicting postoperative fever following flexible ureteroscopic lithotripsy: a propensity score matching analysis.World journal of urology · 2021Article
- Urinary Microbiome: Yin and Yang of the Urinary Tract.Frontiers in cellular and infection microbiology · 2021Review
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundUrinary tract infections are known to be caused by bacteria, but the potential implications of archaea have never been studied in this context.
methodsIn two different university hospital centres we used specific laboratory methods for the detection and culture of archaeal methanogens in 383 urine specimens prospectively collected for diagnosing urinary tract infection (UTI).
findingsMethanobrevibacter smithii was detected by quantitative PCR and sequencing in 34 (9%) of the specimens collected from 34 patients. Escherichia coli, Klebsiella pneumoniae, Enterobacter sp., Enterococcus faecium and mixed cultures were detected along with M. smithii in eighteen, six, three, one and six urine samples, respectively. Interestingly, using our specific culture method for methanogens, we also isolated M. smithii in 31 (91%) of the 34 PCR positive urine samples. Genotyping the 31 isolates using multispacer sequence typing revealed three different genotypes which have been previously reported in intestinal microbiota. Antibiotic susceptibility testing found the 31 isolates to be in vitro susceptible to metronidazole (MIC: 1 mg/L) but resistant to fosfomycin, sulfamethoxazole-trimethoprim, amoxicillin-clavulanate and ofloxacin, commonly used to treat bacterial UTI. Finally, 19 (54%) of the 34 patients in whose urine samples M. smithii was detected were diagnosed with UTIs, including cystitis, pyelonephritis and prostatitis.
interpretationOur results show that M. smithii is part of the urinary microbiota of some individuals and could play a role in community-acquired UTI in association with enteric bacteria. FUND: This study was supported by IHU Méditerranée Infection, Marseille, France.
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