Evidence map›Paper›PMID 31072312›Full record

ArticleBMC bioinformatics2019

NanoDJ: a Dockerized Jupyter notebook for interactive Oxford Nanopore MinION sequence manipulation and genome assembly.

Héctor Rodríguez-Pérez, Tamara Hernández-Beeftink, José M Lorenzo-Salazar, José L Roda-García, Carlos J Pérez-González, Marcos Colebrook, Carlos Flores

Abstract read
In one paragraph

Article in BMC bioinformatics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Bioinformatics of nanopore sequencing.Journal of human genetics · 2020
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Héctor Rodríguez-PérezResearch Unit, Hospital Universitario Nuestra Señora de Candelaria, Universidad de La Laguna, Santa Cruz de Tenerife, Spain.
Tamara Hernández-BeeftinkResearch Unit, Hospital Universitario Nuestra Señora de Candelaria, Universidad de La Laguna, Santa Cruz de Tenerife, Spain.
José M Lorenzo-SalazarGenomics Division, Instituto Tecnológico y de Energías Renovables (ITER), Santa Cruz de Tenerife, Spain.
José L Roda-GarcíaDepartamento de Ingeniería Informática y de Sistemas, Universidad de La Laguna, Santa Cruz de Tenerife, Spain.
Carlos J Pérez-GonzálezDepartamento de Matemáticas, Estadística e Investigación Operativa, Universidad de La Laguna, Santa Cruz de Tenerife, Spain.
Marcos ColebrookDepartamento de Ingeniería Informática y de Sistemas, Universidad de La Laguna, Santa Cruz de Tenerife, Spain. mcolesan@ull.edu.es.
Carlos FloresResearch Unit, Hospital Universitario Nuestra Señora de Candelaria, Universidad de La Laguna, Santa Cruz de Tenerife, Spain. cflores@ull.edu.es.ORCID http://orcid.org/0000-0001-5352-069X

Funding

Agreement with Instituto Tecnológico y de Energías Renovables (ITER) to strengthen scientific and technological education, training, research, development and innovation in Genomics, Personalized Medicine and Biotechnology OA17/008Area Tenerife 2030, Cabildo de Tenerife CGIEU0000219140Instituto de Salud Carlos III rom the Spanish Ministry of Economy and Competitiveness, co-financed by the European Regional Development Funds 'A way of making Europe' from the European Union PI14/00844Instituto de Salud Carlos III rom the Spanish Ministry of Economy and Competitiveness, co-financed by the European Regional Development Funds 'A way of making Europe' from the European Union PI17/00610Spanish Ministry of Economy and Competitiveness MTM2016-74877-PSpanish Ministry of Science, Innovation and Universities RTC-2017-6471-1
6 · The paper itself

Abstract

backgroundThe Oxford Nanopore Technologies (ONT) MinION portable sequencer makes it possible to use cutting-edge genomic technologies in the field and the academic classroom.

resultsWe present NanoDJ, a Jupyter notebook integration of tools for simplified manipulation and assembly of DNA sequences produced by ONT devices. It integrates basecalling, read trimming and quality control, simulation and plotting routines with a variety of widely used aligners and assemblers, including procedures for hybrid assembly.

conclusionsWith the use of Jupyter-facilitated access to self-explanatory contents of applications and the interactive visualization of results, as well as by its distribution into a Docker software container, NanoDJ is aimed to simplify and make more reproducible ONT DNA sequence analysis. The NanoDJ package code, documentation and installation instructions are freely available at https://github.com/genomicsITER/NanoDJ .

Indexed as

NanoporesGenomicsHigh-Throughput Nucleotide SequencingSequence Analysis, DNADockerGenome analysisJupyterNanopore sequencing

Identifiers

PMID31072312
PMCPMC6509807

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.