ArticlePloS one2019
Volatile anesthetics affect macrophage phagocytosis.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 31 citations in OpenAlex.
- Inhalation Anesthetics Play a Janus-Faced Role in Self-Renewal and Differentiation of Stem Cells.Biomolecules · 2024Review
- Mechanism of isoflurane‑mediated breast cancer growthOncology letters · 2024Article
- Comparison between sevoflurane and propofol on immunomodulation in anFrontiers in medicine · 2023Article
- Effect of Propofol versus Sevoflurane Anesthesia on Acute Kidney Injury after Lung Transplantation Surgery: A Prospective Randomized Controlled Trial.Journal of clinical medicine · 2022Article
- Total intravenous anesthesia for liver resections: anesthetic implications and safety.Korean journal of anesthesiology · 2022Article
- Roles of Rufy3 in experimental subarachnoid hemorrhage-induced early brain injury via accelerating neuronal axon repair and synaptic plasticity.Molecular brain · 2022Article
- Isoflurane attenuates sepsis-associated lung injury.Biochemical and biophysical research communications · 2022Article
- Robustness of Propofol and Sevoflurane on the Perioperative Immune Function of Patients Undergoing Laparoscopic Radical Nephrectomy.Journal of oncology · 2022Article
- Effects of propofol on macrophage activation and function in diseases.Frontiers in pharmacology · 2022Review
- The benefits of propofol on cancer treatment: Decipher its modulation code to immunocytes.Frontiers in pharmacology · 2022Review
- Epac-2 ameliorates spontaneous colitis in Il-10Journal of cellular and molecular medicine · 2022Article
- Anesthetics isoflurane and sevoflurane attenuate flagellin-mediated inflammation in the lung.Biochemical and biophysical research communications · 2021Article
- Article
- Molecular Aspects of Volatile Anesthetic-Induced Organ Protection and Its Potential in Kidney Transplantation.International journal of molecular sciences · 2021Review
- The Role of Anesthetic Management in Surgical Site Infections After Pediatric Intestinal Surgery.The Journal of surgical research · 2021Article
- The Role of General Anesthetic Drug Selection in Cancer Outcome.BioMed research international · 2021Review
- Mechanistic consideration of the effect of perioperative volatile anesthetics on phagocytes.Clinical immunology (Orlando, Fla.) · 2021Review
- Surgical Site Infections and Perioperative Optimization of Host Immunity by Selection of Anesthetics.BioMed research international · 2021Review
- The effect of anesthetics on toll like receptor 9.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- Volatile Anesthetic Sevoflurane Attenuates Toll-Like Receptor 1/2 Activation.Anesthesia and analgesia · 2020Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 3 countries.
Funding
Abstract
backgroundPerioperative infections, particularly surgical site infections pose significant morbidity and mortality. Phagocytosis is a critical step for microbial eradication. We examined the effect of commonly used anesthetics on macrophage phagocytosis and its mechanism.
methodsThe effect of anesthetics (isoflurane, sevoflurane, propofol) on macrophage phagocytosis was tested using RAW264.7 mouse cells, mouse peritoneal macrophages, and THP-1 human cells. Either opsonized sheep erythrocytes or fluorescent labeled Escherichia coli were used as phagocytic objects. The activation of Rap1, a critical protein in phagocytosis was assessed using the active Rap1 pull-down and detection kit. To examine anesthetic binding site(s) on Rap1, photolabeling experiments were performed using azi-isoflurane and azi-sevoflurane. The alanine scanning mutagenesis of Rap1 was performed to assess the role of anesthetic binding site in Rap1 activation and phagocytosis.
resultsMacrophage phagocytosis was significantly attenuated by the exposure of isoflurane (50% reduction by 1% isoflurane) and sevoflurane (50% reduction by 1.5% sevoflurane), but not by propofol. Photolabeling experiments showed that sevoflurane directly bound to Rap1. Mutagenesis analysis demonstrated that the sevoflurane binding site affected Rap1 activation and macrophage phagocytosis.
conclusionsWe showed that isoflurane and sevoflurane attenuated macrophage phagocytosis, but propofol did not. Our study showed for the first time that sevoflurane served as a novel small GTPase Rap1 inhibitor. The finding will further enrich our understanding of yet-to-be determined mechanism of volatile anesthetics and their off-target effects. The sevoflurane binding site was located outside the known Rap1 functional sites, indicating the discovery of a new functional site on Rap1 and this site would serve as a pocket for the development of novel Rap1 inhibitors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.