Evidence map›Paper›PMID 31069776›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2019

In Vivo Measurement of Redox-Regulated TG2 Activity.

Arek V Melkonian, Nielson Weng, Brad A Palanski, Chaitan Khosla

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
3.1field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Structural and mechanistic analysis of CaProceedings of the National Academy of Sciences of the United States of America · 2024
    Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Arek V MelkonianDepartment of Chemical Engineering, Stanford University, Stanford, CA, USA.
Nielson WengSchool of Medicine, Stanford University, Stanford, CA, USA.
Brad A PalanskiDepartment of Chemistry, Stanford University, Stanford, CA, USA.
Chaitan KhoslaDepartment of Chemical Engineering, Stanford University, Stanford, CA, USA. khosla@stanford.edu.
Stanford University · US

Funding

Role of Transglutaminase 2 in Celiac SprueR01DK063158 · NIDDK · STANFORD UNIVERSITY · PI CHAITAN KHOSLA · 2003 to 2026
$11.2M
Preclinical Validation of Transglutaminase 2 as a Novel Target for Celiac DiseaseR01DK100619 · NIDDK · STANFORD UNIVERSITY · PI KHOSLA, CHAITAN · 2014 to 2018
$2.3M
NIDDK NIH HHS R01 DK063158NIDDK NIH HHS R01 DK100619
6 · The paper itself

Abstract

Transglutaminase 2 (TG2) is a ubiquitous mammalian enzyme that is implicated in a variety of physiological processes and human diseases. Normally, extracellular TG2 is catalytically dormant due to formation of an allosteric disulphide bond between Cys370 and 371 of the enzyme. In this protocol, we describe a method to reduce this disulphide bond in living mice and to monitor the resulting in vivo TG2 activity. Briefly, exogenous thioredoxin-1 protein (TRX) is prepared and administered as a specific, physiologically relevant reductant of the Cys370-371 disulphide along with the small molecule 5-biotinamidopentylamine (5-BP) as a TG2 activity probe. Tissue cryosections are then analyzed by immunohistochemistry to ascertain the extent of 5-BP incorporation, which serves as a record of the redox state of TG2 in vivo. This protocol focuses on the modulation and measurement of TG2 in the small intestine, but we encourage investigators to evaluate it in their organ(s) of interest.

Indexed as

AminesAnimalsBiotinDisulfidesGTP-Binding ProteinsHumansMiceMolecular BiologyOxidation-ReductionProtein Glutamine gamma Glutamyltransferase 2ThioredoxinsTransglutaminases5-(biotinamido)pentylamineAminesBiotinDisulfidesGTP-Binding ProteinsProtein Glutamine gamma Glutamyltransferase 2ThioredoxinsTransglutaminasesActivationDisulphideInhibitionIn vivoMouse studyProbeRedoxSmall intestineThioredoxinTransglutaminase

Identifiers

PMID31069776
PMCPMC6852785
OpenAlexW2943976246

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.