Evidence map›Paper›PMID 31069610›Full record

ArticleIn vitro cellular & developmental biology. Animal2019

Cyclin-dependent kinase 5 regulates proliferation, migration, tyrosinase activity, and melanin production in B16-F10 melanoma cells via the essential regulator p-CREB.

Xiuqing Li, Ruifang Wang, Junzhen Zhang, Shanshan Yang, Kaiyuan Ji, Bin Du, Xuexian Liu, Bo Liu, Shuhui Qi, Qiong Jia and 1 more

Abstract read
PubMed Publisher
In one paragraph

Article in In vitro cellular & developmental biology. Animal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.1field-weighted citation impact, top 54% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 2 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Xiuqing LiCollege of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Mingxian South Road, Taigu, 030801, China.ORCID http://orcid.org/0000-0003-2797-2697
Ruifang WangCollege of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Mingxian South Road, Taigu, 030801, China.
Junzhen ZhangCollege of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Mingxian South Road, Taigu, 030801, China.
Shanshan YangCollege of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Mingxian South Road, Taigu, 030801, China.
Kaiyuan JiCollege of Animal Science and Veterinary Medicine, Anhui Agricultural University, Changjiang West Road, Hefei, 230036, China.
Bin DuCollege of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Mingxian South Road, Taigu, 030801, China.
Xuexian LiuCollege of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Mingxian South Road, Taigu, 030801, China.
Bo LiuCollege of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Mingxian South Road, Taigu, 030801, China.
Shuhui QiCollege of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Mingxian South Road, Taigu, 030801, China.
Qiong JiaCollege of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Mingxian South Road, Taigu, 030801, China.
Ruiwen FanCollege of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Mingxian South Road, Taigu, 030801, China. 13453216804@163.com.
Shanxi Agricultural University · CNAnhui Agricultural University · CN

Funding

Shanxi Scholarship Council of China 2017-072
6 · The paper itself

Abstract

Melanoma is an aggressive cancer with increasing incidence and a growing lifetime risk that arises from normal melanocytes or their precursors. A thorough understanding of the molecular mechanism of melanomagenesis and melanoma biology is essential for the diagnosis, prognostication, and therapy of melanoma. Cyclin-dependent protein kinase 5 (Cdk5) is one of the proteins highly expressed in B16-F10 melanoma cells that controls melanoma cell motility, invasiveness, and metastatic spread and might be a promising novel therapeutic target. The effect of Cdk5 on proliferation and migration, which are important for carcinogenesis, has not been reported. In the current study, we found that siRNA-mediated knockdown of Cdk5 in B16-F10 melanoma cells inhibited melanoma cell proliferation through downregulation of the CaMK4-p-CREB pathway, inhibited migration through downregulation of p-CREB, integrin beta 1, and integrin beta 5, and also inhibited tyrosinase activity and melanin production through p-CREB-MITF regulation. The results indicate that Cdk5 controls melanoma development, with an essential regulatory role for p-CREB.

Indexed as

AnimalsCell Line, TumorCell MovementCell ProliferationCyclic AMP Response Element-Binding ProteinCyclin-Dependent Kinase 5Gene Expression Regulation, EnzymologicGene Expression Regulation, NeoplasticHumansMelaninsMelanoma, ExperimentalMiceMonophenol MonooxygenaseCDK5 protein, humanCdk5 protein, mouseCreb1 protein, mouseCyclic AMP Response Element-Binding ProteinCyclin-Dependent Kinase 5MelaninsMonophenol MonooxygenaseCdk5MelanomaMigrationPhosphorated CREB (p-CREB)Proliferation

Identifiers

PMID31069610
OpenAlexW2944267029

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.