ArticleHorticulture research2019
Optimization and standardization of transient expression assays for gene functional analyses in strawberry fruits.
Article in Horticulture research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- A signaling pathway mediating abscisic acid-induced jasmonic acid biosynthesis during strawberry ripening.Journal of integrative plant biology · 2026Article
- Agroinfiltration technique for elucidating the functions of strawberry genes in Fragaria vesca.Scientific reports · 2025Article
- A telomere-to-telomere phased genome of an octoploid strawberry reveals a receptor kinase conferring anthracnose resistance.GigaScience · 2025Article
- Article
- Improvement and application of vacuum-infiltration system in tomato.Horticulture research · 2024Article
- Identification of MYB Transcription Factor, a Regulator Related to Hydrolysable Tannin Synthesis inPlants (Basel, Switzerland) · 2024Article
- Critical parameters for robustPlant direct · 2024Article
- Transient Expression Assay and Microscopic Observation in Kumquat Fruit.Bio-protocol · 2024Article
- Non-climacteric fruit development and ripening regulation: 'the phytohormones show'.Journal of experimental botany · 2023Review
- Competition between FLS and DFR regulates the distribution of flavonols and proanthocyanidins inFrontiers in plant science · 2023Article
- Hexokinase1: A glucose sensor involved in drought stress response and sugar metabolism depending on its kinase activity in strawberry.Frontiers in plant science · 2023Article
- An LTR retrotransposon in the promoter of aHorticulture research · 2022Article
- The protein kinase FvRIPK1 regulates plant morphogenesis by ABA signaling using seed genetic transformation in strawberry.Frontiers in plant science · 2022Article
- Signal transduction in non-climacteric fruit ripening.Horticulture research · 2022Article
- Do Non-climacteric Fruits Share a Common Ripening Mechanism of Hormonal Regulation?Frontiers in plant science · 2022Review
- A Method for Assaying of Protein Kinase Activity In Vivo and Its Use in Studies of Signal Transduction in Strawberry Fruit Ripening.International journal of molecular sciences · 2021Article
- An effector-reporter system to study cellular signal transduction in strawberry fruit (Fragaria ananassa).Horticulture research · 2021Article
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15 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Strawberry is increasingly used as a model plant for research on fruit growth and development. The transient gene manipulation (TGM) technique is widely used to determine the function of plant genes, including those in strawberry fruits. However, its reliable application for the precise identification of gene function has been difficult owing to the lack of conditional optimization. In this study, we found that successful transient gene manipulation requires optimization, with the vector type, temperature, and fruit developmental stage being three major factors determining success. Notably, we found that transient gene manipulation was feasible only from the large green fruit stage onwards, making it especially suitable for identifying genes involved in strawberry fruit ripening. Furthermore, we established a method called percentage difference of phenotype (PDP), in which the functional effect of a gene could be precisely and efficiently identified in strawberry fruits. This method can be used to estimate the functional effect of a gene as a value from 0 to 100%, such that different genes can be quantitatively compared for their relative abilities to regulate fruit ripening. This study provides a useful tool for accelerating research on the molecular basis of strawberry fruit ripening.
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