ArticleNature communications2019
Tissue-specific (ts)CRISPR as an efficient strategy for in vivo screening in Drosophila.
Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 116 citations in OpenAlex.
- Patronin facilitates neurite remodeling via epithelial-to-neuronal signaling.Cell communication and signaling : CCS · 2026Article
- Stage- and tissue-specific gene editing using 4-OHT-inducible Cas9 in whole organism.The Journal of cell biology · 2026Article
- Improved in vivo gene knockout with high specificity using multiplexed Cas12a sgRNAs.Nature communications · 2026Article
- ALife science alliance · 2025Article
- Methods and applications of in vivo CRISPR screening.Nature reviews. Genetics · 2025Review
- Single-cell transcriptional landscapes of Aedes aegypti midgut and fat body after a bloodmeal.Cell genomics · 2025Article
- Glia-derived noncanonical fatty acid binding protein modulates brain lipid storage and clearance.Science advances · 2025Article
- Glycolytic disruption restricts Drosophila melanogaster larval growth via the cytokine Upd3.PLoS genetics · 2025Article
- Astrocyte-dependent local neurite pruning in Beat-Va neurons.The Journal of cell biology · 2025Article
- Article
- Tissue-specific knockout in the Drosophila neuromuscular system reveals ESCRT's role in formation of synapse-derived extracellular vesicles.PLoS genetics · 2024Article
- A genetic screen of transcription factors in the Drosophila melanogaster abdomen identifies novel pigmentation genes.G3 (Bethesda, Md.) · 2024Article
- Future avenues inLearning & memory (Cold Spring Harbor, N.Y.) · 2024Review
- Identification of secretory autophagy as a mechanism modulating activity-induced synaptic remodeling.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- A drug stabilizable GAL80Scientific reports · 2024Article
- High-throughput and genome-scale targeted mutagenesis using CRISPR in a nonmodel multicellular organism,Genome research · 2024Article
- tsCRISPR based identification of Rab proteins required for the recycling ofFrontiers in cell and developmental biology · 2024Article
- Generation of Mutants from the 57B Region ofGenes · 2023Article
- Tissue-specific knockout inbioRxiv : the preprint server for biology · 2023Article
- A temperature-tolerant CRISPR base editor mediates highly efficient and precise gene editing inScience advances · 2023Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
Gene editing by CRISPR/Cas9 is commonly used to generate germline mutations or perform in vitro screens, but applicability for in vivo screening has so far been limited. Recently, it was shown that in Drosophila, Cas9 expression could be limited to a desired group of cells, allowing tissue-specific mutagenesis. Here, we thoroughly characterize tissue-specific (ts)CRISPR within the complex neuronal system of the Drosophila mushroom body. We report the generation of a library of gRNA-expressing plasmids and fly lines using optimized tools, which provides a valuable resource to the fly community. We demonstrate the application of our library in a large-scale in vivo screen, which reveals insights into developmental neuronal remodeling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.