Evidence map›Paper›PMID 31067783›Full record

ArticleGenes2019

Full-Length Multi-Barcoding: DNA Barcoding from Single Ingredient to Complex Mixtures.

Peng Zhang, Chunsheng Liu, Xiasheng Zheng, Lan Wu, Zhixiang Liu, Baosheng Liao, Yuhua Shi, Xiwen Li, Jiang Xu, Shilin Chen

Abstract read
In one paragraph

Article in Genes, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Review
  6. Article
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Peng ZhangSchool of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China. zheng.x.s1987@163.com.
Chunsheng LiuSchool of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China. max_liucs@263.net.
Xiasheng ZhengGuangdong Provincial Key Laboratory of New Drug Development and Research of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China. zheng.x.s1987@163.com.
Lan WuKey Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of ChineseMateria Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. lwu@icmm.ac.cn.
Zhixiang LiuKey Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of ChineseMateria Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. zhixiangliu88@163.com.
Baosheng LiaoKey Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of ChineseMateria Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. swjs082lbs@126.com.
Yuhua ShiKey Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of ChineseMateria Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. yhshi@icmm.ac.cn.
Xiwen LiKey Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of ChineseMateria Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. xwli@icmm.ac.cn.
Jiang XuKey Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of ChineseMateria Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. jxu@icmm.ac.cn.
Shilin ChenKey Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of ChineseMateria Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. slchen@icmm.ac.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

DNA barcoding has been used for decades, although it has mostly been applied to somesingle-species. Traditional Chinese medicine (TCM), which is mainly used in the form ofcombination-one type of the multi-species, identification is crucial for clinical usage.Next-generation Sequencing (NGS) has been used to address this authentication issue for the pastfew years, but conventional NGS technology is hampered in application due to its short sequencingreads and systematic errors. Here, a novel method, Full-length multi-barcoding (FLMB) vialong-read sequencing, is employed for the identification of biological compositions in herbalcompound formulas in adequate and well controlled studies. By directly sequencing the full-lengthamplicons of ITS2 and

Indexed as

Chloroplast ProteinsDNA, IntergenicDNA, PlantDNA, RibosomalDrug CombinationsDrugs, Chinese HerbalSequence Analysis, DNAChloroplast ProteinsDNA, IntergenicDNA, PlantDNA, RibosomalDrug CombinationsDrugs, Chinese Herbalfructus schizandrae, radix ginseng, radix ophiopogonis drug combinationDNA barcodingITS2multi-species mixturesnext-generation sequencingSheng-Mai-San (SMS)single-molecule real-time (SMRT)

Identifiers

PMID31067783
PMCPMC6562688

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.