ArticleInternational journal of molecular sciences2019
C1 Compound Biosensors: Design, Functional Study, and Applications.
Article in International journal of molecular sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 21 citations in OpenAlex.
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- Sustainable One-Pot Electrochemical Approach for Entrapment of Multi-Enzymes and Biocompatible Redox Mediator.ChemPlusChem · 2025Article
- Metagenomic interrogation of urban Superfund site reveals antimicrobial resistance reservoir and bioremediation potential.Journal of applied microbiology · 2025Article
- Directed evolution of a neutrophilic and mesophilic methanol dehydrogenase based on high-throughput and accurate measurement of formaldehyde.Synthetic and systems biotechnology · 2023Article
- Isolation and Characterization of Homologically Expressed Methanol Dehydrogenase fromInternational journal of molecular sciences · 2022Article
- Approaches to Formaldehyde Measurement: From Liquid Biological Samples to Cells and Organisms.International journal of molecular sciences · 2022Review
- Strategies for Improving Small-Molecule Biosensors in Bacteria.Biosensors · 2022Review
- Biosensor-Based Directed Evolution of Methanol Dehydrogenase fromInternational journal of molecular sciences · 2021Article
- Genetically Encoded Biosensor-Based Screening for Directed Bacteriophage T4 Lysozyme Evolution.International journal of molecular sciences · 2020Article
- Sensitive and Rapid Phenotyping of Microbes With Soluble Methane Monooxygenase Using a Droplet-Based Assay.Frontiers in bioengineering and biotechnology · 2020Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
The microbial assimilation of one-carbon (C1) gases is a topic of interest, given that products developed using this pathway have the potential to act as promising substrates for the synthesis of valuable chemicals via enzymatic oxidation or C-C bonding. Despite extensive studies on C1 gas assimilation pathways, their key enzymes have yet to be subjected to high-throughput evolution studies on account of the lack of an efficient analytical tool for C1 metabolites. To address this challenging issue, we attempted to establish a fine-tuned single-cell-level biosensor system constituting a combination of transcription factors (TFs) and several C1-converting enzymes that convert target compounds to the ligand of a TF. This enzymatic conversion broadens the detection range of ligands by the genetic biosensor systems. In this study, we presented new genetic enzyme screening systems (GESSs) to detect formate, formaldehyde, and methanol from specific enzyme activities and pathways, named FA-GESS, Frm-GESS, and MeOH-GESS, respectively. All the biosensors displayed linear responses to their respective C1 molecules, namely, formate (1.0-250 mM), formaldehyde (1.0-50 μM), and methanol (5-400 mM), and they did so with high specificity. Consequently, the helper enzymes, including formaldehyde dehydrogenase and methanol dehydrogenase, were successfully combined to constitute new versatile combinations of the C1-biosensors.
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Registered trials
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