ArticleJournal of tissue engineering and regenerative medicine2019
Microparticles for controlled growth differentiation factor 6 delivery to direct adipose stem cell-based nucleus pulposus regeneration.
Article in Journal of tissue engineering and regenerative medicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 25 citations in OpenAlex.
- From Bench to Bedside: Translational Barriers in Mesenchymal Stem Cell Therapy for Intervertebral Disc Degeneration.Bioengineering (Basel, Switzerland) · 2026Review
- Engineering Intervertebral Disc Regeneration: Biomaterials, Cell Sources and Animal Models.Cell proliferation · 2025Review
- Application of Endogenous Stem Cells in the Repair of Annulus Fibrosus Injury of Intervertebral Discs.Stem cells international · 2025Review
- Injectable smart-blended hydrogel cross-linked with Vanillin to accelerate differentiation of intervertebral disc-derived stem cells (IVDSCs) for promoting degenerative nucleolus pulposus in a rat model.Inflammopharmacology · 2024Article
- Application trends and strategies of hydrogel delivery systems in intervertebral disc degeneration: A bibliometric review.Materials today. Bio · 2024Review
- Stem cell therapy for degenerative disc disease: Bridging the gap between preclinical promise and clinical potential.Biomolecules & biomedicine · 2024Review
- Article
- Decellularized nucleus pulposus matrix/chitosan hybrid hydrogel combined with nucleus pulposus stem cells and GDF5-loaded microspheres for intervertebral disc degeneration prevention.Molecular medicine (Cambridge, Mass.) · 2024Article
- Review
- Codelivery of TGF-β1 and anti-miR-141 by PLGA microspheres inhibits progression of intervertebral disc degeneration.Journal of orthopaedic surgery and research · 2023Article
- Precision medicine strategies for spinal degenerative diseases: Injectable biomaterials with in situ repair and regeneration.Materials today. Bio · 2022Review
- New Hope for Treating Intervertebral Disc Degeneration: Microsphere-Based Delivery System.Frontiers in bioengineering and biotechnology · 2022Review
- Application of single and cooperative different delivery systems for the treatment of intervertebral disc degeneration.Frontiers in bioengineering and biotechnology · 2022Review
- Droplet microfluidic devices for organized stem cell differentiation into germ cells: capabilities and challenges.Biophysical reviews · 2021Review
- Multiscale Regulation of the Intervertebral Disc: Achievements in Experimental, In Silico, and Regenerative Research.International journal of molecular sciences · 2021Review
- Applications of Functionalized Hydrogels in the Regeneration of the Intervertebral Disc.BioMed research international · 2021Review
- Regenerative Response of Degenerate Human Nucleus Pulposus Cells to GDF6 Stimulation.International journal of molecular sciences · 2020Article
- Cell and Gene Therapy for Spine Regeneration: Mammalian Protein Production Platforms for Overproduction of Therapeutic Proteins and Growth Factors.Neurosurgery clinics of North America · 2020Review
- Microparticles for controlled growth differentiation factor 6 delivery to direct adipose stem cell-based nucleus pulposus regeneration.Journal of tissue engineering and regenerative medicine · 2019Article
- High BMPR2 expression leads to enhanced SMAD1/5/8 signalling and GDF6 responsiveness in human adipose-derived stem cells: implications for stem cell therapies for intervertebral disc degeneration.Journal of tissue engineeringArticle
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Currently, there is no effective long-term treatment for intervertebral disc (IVD) degeneration, making it an attractive candidate for regenerative therapies. Hydrogel delivery of adipose stem cells (ASCs) in combination with controlled release of bioactive molecules is a promising approach to halt IVD degeneration and promote regeneration. Growth differentiation factor 6 (GDF6) can induce ASC differentiation into anabolic nucleus pulposus (NP) cells and hence holds promise for IVD regeneration. Here, we optimised design of novel poly(DL-lactic acid-co-glycolic acid) (PLGA)-polyethylene glycol-PLGA microparticles to control GDF6 delivery and investigated effect of released GDF6 on human ASCs differentiation to NP cells. Recombinant human (rh)GDF6 was loaded into microparticles and total protein and rhGDF6 release assessed. The effect of microparticle loading density on distribution and gel formation was investigated through scanning electron microscopy. ASC differentiation to NP cells was examined after 14 days in hydrogel culture by quantitative polymerase chain reaction, histological, and immunohistochemical staining in normoxic and IVD-like hypoxic conditions. RhGDF6 microparticles were distributed throughout gels without disrupting gelation and controlled rhGDF6 release over 14 days. Released GDF6 significantly induced NP differentiation of ASCs, with expression comparable with or exceeding media supplemented rhGDF6. Microparticle-delivered rhGDF6 also up-regulated sulphated glycosaminoglycan and aggrecan secretion in comparison with controls. In hypoxia, microparticle-delivered rhGDF6 continued to effectively induce NP gene expression and aggrecan production. This study demonstrates the effective encapsulation and controlled delivery of rhGDF6, which maintained its activity and induced ASC differentiation to NP cells and synthesis of an NP-like matrix suggesting suitability of microparticles for controlled growth factor release in regenerative strategies for treatment of IVD degeneration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.