Evidence map›Paper›PMID 31066288›Full record

ArticleCell transplantation2019

Immunomodulation with Human Umbilical Cord Blood Stem Cells Ameliorates Ischemic Brain Injury - A Brain Transcriptome Profiling Analysis.

Maple L Shiao, Ce Yuan, Andrew T Crane, Joseph P Voth, Mario Juliano, Laura L Hocum Stone, Zhenghong Nan, Ying Zhang, Nicole Kuzmin-Nichols, Paul R Sanberg and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell transplantation, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
1.6field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 23 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

Maple L Shiao1 Department of Neurosurgery, University of Minnesota, Minneapolis, USA.
Ce Yuan2 Graduate Program in Bioinformatics and Computational Biology, University of Minnesota, Minneapolis, USA.
Andrew T Crane1 Department of Neurosurgery, University of Minnesota, Minneapolis, USA.
Joseph P Voth1 Department of Neurosurgery, University of Minnesota, Minneapolis, USA.
Mario Juliano1 Department of Neurosurgery, University of Minnesota, Minneapolis, USA.
Laura L Hocum Stone1 Department of Neurosurgery, University of Minnesota, Minneapolis, USA.
Zhenghong Nan1 Department of Neurosurgery, University of Minnesota, Minneapolis, USA.
Ying Zhang4 Minnesota Supercomputing Institute, University of Minnesota, Minneapolis, USA.
Nicole Kuzmin-Nichols5 Saneron CCEL Therapeutics Inc, Tampa, USA.
Paul R Sanberg6 Center for Brain Repair and Department of Neurosurgery, Morsani College of Medicine, University of South Florida, Tampa, USA.
Andrew W Grande1 Department of Neurosurgery, University of Minnesota, Minneapolis, USA.
Walter C Low1 Department of Neurosurgery, University of Minnesota, Minneapolis, USA.
University of Minnesota · USSaneron CCEL Therapeutics (United States) · USUniversity of South Florida · US

Funding

Transplantation of Umbilical Cord Blood Stem Cells in Ischemic Brain InjuryR41NS056626 · NINDS · SANERON CCEL THERAPEUTICS, INC. · PI KUZMIN-NICHOLS, NICOLE A · 2008 to 2008
$96k
NINDS NIH HHS R41 NS056626
6 · The paper itself

Abstract

Our group previously demonstrated that administration of a CD34-negative fraction of human non- hematopoietic umbilical cord blood stem cells (UCBSC) 48 h after ischemic injury could reduce infarct volume by 50% as well as significantly ameliorate neurological deficits. In the present study, we explored possible mechanisms of action using next generation RNA sequencing to analyze the brain transcriptome profiles in rats with ischemic brain injury following UCBSC therapy. Two days after ischemic injury, rats were treated with UCBSC. Five days after administration, total brain mRNA was then extracted for RNAseq analysis using Illumina Hiseq 2000. We found 275 genes that were significantly differentially expressed after ischemic injury compared with control brains. Following UCBSC treatment, 220 of the 275 differentially expressed genes returned to normal levels. Detailed analysis of these altered transcripts revealed that the vast majority were associated with activation of the immune system following cerebral ischemia which were normalized following UCBSC therapy. Major alterations in gene expression profiles after ischemia include blood-brain-barrier breakdown, cytokine production, and immune cell infiltration. These results suggest that UCBSC protect the brain following ischemic injury by down regulating the aberrant activation of innate and adaptive immune responses.

Indexed as

AnimalsBlood-Brain BarrierComputational BiologyHumansHuman Umbilical Vein Endothelial CellsImmunomodulationMacrophagesMicrogliaRatsRats, Sprague-DawleyStem Cellshuman umbilical cord bloodimmunomodulationmacrophagemicroglianeuroinflammationstem cellsstroke

Identifiers

PMID31066288
PMCPMC6719500
OpenAlexW2944352587

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.