ArticleJournal of biomedical optics2019
Nanoparticle-based assay for detection of S100P mRNA using surface-enhanced Raman spectroscopy.
Article in Journal of biomedical optics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 25 citations in OpenAlex.
- Extraoral Detection of Biomarkers and Pathogens in Saliva: Comprehensive, Panoramic Review.Biosensors · 2026Review
- Plasmonics-enhanced spikey nanorattle-based biosensor for direct SERS detection of mRNA cancer biomarkers.Analytical and bioanalytical chemistry · 2024Article
- Advances in Raman spectroscopy for characterising oral cancer and oral potentially malignant disorders.Expert reviews in molecular medicine · 2024Review
- The Integration of Gold Nanoparticles into Dental Biomaterials as a Novel Approach for Clinical Advancement: A Narrative Review.Journal of functional biomaterials · 2024Review
- S100 proteins in head and neck squamous cell carcinoma (Review).Oncology letters · 2023Review
- Nucleotide detection mechanism and comparison based on low-dimensional materials: A review.Frontiers in bioengineering and biotechnology · 2023Review
- Gold nanomaterials for oral cancer diagnosis and therapy: Advances, challenges, and prospects.Materials today. Bio · 2022Article
- Raman Spectroscopy: A Potential Diagnostic Tool for Oral Diseases.Frontiers in cellular and infection microbiology · 2022Review
- Advances in the Diagnosis, Monitoring, and Progression of Oral Cancer through Saliva: An Update.BioMed research international · 2022Article
- Malignancies and Biosensors: A Focus on Oral Cancer Detection through Salivary Biomarkers.Biosensors · 2021Review
- Photonics of human saliva: potential optical methods for the screening of abnormal health conditions and infections.Biophysical reviews · 2021Review
- Vibrational Spectroscopy for Identification of Metabolites in Biologic Samples.Molecules (Basel, Switzerland) · 2020Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The focus of this work is toward the development of a point-of-care (POC) handheld technology for the noninvasive early detection of salivary biomarkers. The initial of focus was the detection and quantification of S100 calcium-binding protein P (S100P) mRNA found in whole saliva for use as a potential biomarker for oral cancer. Specifically, a surface-enhanced Raman spectroscopy (SERS)-based approach and assay were designed, developed, and tested for sensitive and rapid detection of S100P mRNA. Gold nanoparticles (AuNPs) were conjugated with oligonucleotides and malachite green isothiocyanate was then used as a Raman reporter molecule. The hybridization of S100P target to DNA-conjugated AuNPs in sandwich assay format in both free solution and a vertical flow chip (VFC) was confirmed using a handheld SERS system. The detection limit of the SERS-based assay in free solution was determined to be 1.1 nM, whereas on the VFC the detection limit was observed to be 10 nM. SERS-based VFCs were also used to quantify the S100P mRNA from saliva samples of oral cancer patients and a healthy group. The result indicated that the amount of S100P mRNA detected for the oral cancer patients is three times higher than that of a healthy group.
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Registered trials
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