Evidence map›Paper›PMID 31065868›Full record

ArticleMikrochimica acta2019

Fluorometric determination of RNase H via a DNAzyme conjugated to reduced graphene oxide, and its application to screening for inhibitors and activators.

Chunyi Tong, Ting Zhou, Chuan Zhao, Liqun Yuan, Ying Xu, Bin Liu, Jialong Fan, Dan Li, Aiguo Zhu

Abstract read
PubMed Publisher
In one paragraph

Article in Mikrochimica acta, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 14 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Chunyi TongCollege of Biology, Hunan University, Changsha, 410082, China.
Ting ZhouCollege of Biology, Hunan University, Changsha, 410082, China.
Chuan ZhaoCollege of Biology, Hunan University, Changsha, 410082, China.
Liqun YuanPeople's Hospital of Hunan Province, Changsha, 410082, China.
Ying XuInstitute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, 410008, People's Republic of China.
Bin LiuCollege of Biology, Hunan University, Changsha, 410082, China. binliu2001@hotmail.com.ORCID 0000-0001-5574-3547
Jialong FanCollege of Biology, Hunan University, Changsha, 410082, China.
Dan LiCollege of Biology, Hunan University, Changsha, 410082, China.
Aiguo ZhuInstitute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, 410008, People's Republic of China. zhuaiguo@caas.cn.
Hunan University · CNInstitute of Bast Fiber Crops · CNHunan Provincial People's Hospital · CN

Funding

National Natural Science Foundation of China 81501218, 81673579, 81874369 and 31672457Natural Science Foundation of Hunan Province h14JJ2049
6 · The paper itself

Abstract

A new fluorometric method is delineated for the detection of RNase H activity by combining DNAzyme with reduced graphene oxide (rGO). In the absence of RNase H, the fluorescence of FAM-labeled probe is quenched due to the strong adsorption on the rGO. The presence of RNase H can release the active DNAzyme from the DNA-RNA chimeric strand. This triggers the cleavage of the signal probe at the rA site with the help of the cofactor Mg

Indexed as

Cell Line, TumorDNA, CatalyticDNA ProbesEnzyme InhibitorsFluoresceinsFluorescent DyesGraphiteHumansLimit of DetectionRibonuclease HSpectrometry, FluorescenceDNA, CatalyticDNA ProbesEnzyme InhibitorsFluoresceinsFluorescent Dyesgraphene oxideGraphiteRibonuclease HConcentration-dependent mannerFluorescent probeHepatitis C virusHigh sensitivityKinetic analysisModel dockingNanomaterialQuenching efficiencySignal amplificationTumor cell lines

Identifiers

PMID31065868
OpenAlexW2944813179

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.