ArticleFrontiers in microbiology2019
Development of a Blocking Primer to Inhibit the PCR Amplification of the 18S rDNA Sequences of
Article in Frontiers in microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it, 49 citations in OpenAlex.
- Transforming gastrointestinal helminth parasite identification in vertebrate hosts with metabarcoding: a systematic review.Parasites & vectors · 2024Pooled it
- Research on Quantitative Analysis Technology for Pacific White Shrimp Density Based on Environmental DNA.Ecology and evolution · 2026Article
- Development of PCR Blocking Primers Enabling DNA Metabarcoding Analysis of Dietary Composition in Hematophagous Sea Lamprey.Ecology and evolution · 2025Article
- Contemporary eDNA methods complement conventional microscopy in zooplankton diet studies: Case study with American lobster postlarvae.PloS one · 2025Article
- Interkingdom interactions shape the fungal microbiome of mosquitoes.Animal microbiome · 2024Article
- Improved 18S rDNA profiling of parasite communities in salmonid tissues using a host blocking primer.Parasitology research · 2024Article
- Seeing in the dark: a metagenomic approach can illuminate the drivers of plant disease.Frontiers in plant science · 2024Article
- LNA blockers for improved amplification selectivity.Scientific reports · 2023Article
- Com probe implemented STexS II greatly enhances specificity in SARS-CoV-2 variant detection.Scientific reports · 2023Article
- DNA metabarcoding reveals the dietary profiles of a benthic marine crustacean, Nephrops norvegicus.PloS one · 2023Article
- Porcine fungal mock community analyses: Implications for mycobiome investigations.Frontiers in cellular and infection microbiology · 2023Article
- Molecular Diet Analysis of Adélie Penguins (Biology · 2022Article
- Revealing the composition of the eukaryotic microbiome of oyster spat by CRISPR-Cas Selective Amplicon Sequencing (CCSAS).Microbiome · 2021Article
- Sampling the fish gill microbiome: a comparison of tissue biopsies and swabs.BMC microbiology · 2021Article
- Exploring Prokaryotic and Eukaryotic Microbiomes Helps in Detecting Tick-Borne Infectious Agents in the Blood of Camels.Pathogens (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Diversity analyses of the eukaryotic microorganisms in the gut of marine animals is hampered by the presence of host DNA in the samples. PCR amplification of rRNA genes of eukaryotic microorganisms is inefficient with universal primers targeting 18S rRNA gene when the host DNA is dominant. In this study, we designed several blocking primers to inhibit PCR amplification of rRNA genes of the shrimp
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Registered trials
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