Evidence map›Paper›PMID 31065249›Full record

ArticleFrontiers in microbiology2019

Antigen-Specific Single B Cell Sorting and Monoclonal Antibody Cloning in Guinea Pigs.

Lin Lei, Karen Tran, Yimeng Wang, James J Steinhardt, Yongli Xiao, Chi-I Chiang, Richard T Wyatt, Yuxing Li

Open access · goldAbstract read
In one paragraph

Article in Frontiers in microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
1.3field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 34 citations in OpenAlex.

  1. Review
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  13. Hybridoma technology: is it still useful?Current research in immunology · 2021
    Review
  14. Monoclonal Antibodies Counteract Opioid-Induced Behavioral and Toxic Effects in Mice and Rats.The Journal of pharmacology and experimental therapeutics · 2020
    Article
  15. Article
  16. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 6 institutions in 1 country.

Lin LeiInstitute for Bioscience and Biotechnology Research, University of Maryland, Rockville, MD, United States.
Karen TranIAVI Neutralizing Antibody Center, The Scripps Research Institute, La Jolla, CA, United States.
Yimeng WangInstitute for Bioscience and Biotechnology Research, University of Maryland, Rockville, MD, United States.
James J SteinhardtInstitute for Bioscience and Biotechnology Research, University of Maryland, Rockville, MD, United States.
Yongli XiaoLaboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, United States.
Chi-I ChiangInstitute for Bioscience and Biotechnology Research, University of Maryland, Rockville, MD, United States.
Richard T WyattIAVI Neutralizing Antibody Center, The Scripps Research Institute, La Jolla, CA, United States.
Yuxing LiInstitute for Bioscience and Biotechnology Research, University of Maryland, Rockville, MD, United States.
Advanced Bioscience Laboratories (United States) · USInstitute for Bioscience and Biotechnology ResearchInternational AIDS Vaccine Initiative · USNational Institute of Allergy and Infectious Diseases · USScripps Research Institute · USUniversity of Maryland, Baltimore · US

Funding

Support Component D/Vaccine Discovery, Concept to ClinicUM1AI100663 · NIAID · SCRIPPS RESEARCH INSTITUTE, THE · PI AHMED, RAFI · 2012 to 2018
$131.5M
VirologyP30AI036214 · NIAID · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI David Mitchell Smith · 1994 to 2026
$78.4M
Non-Human PrimatesP01AI104722 · NIAID · SCRIPPS RESEARCH INSTITUTE, THE · PI KARLSSON HEDESTAM, GUNILLA · 2014 to 2018
$14.6M
High-resolution definition of B cell responses to HIV Env for immunogen designR01AI102766 · NIAID · UNIV OF MARYLAND, COLLEGE PARK · PI LI, YUXING · 2013 to 2024
$6.6M
Next Generation Broadly Neutralizing Antibodies to Clear HIV-1 ReservoirR01AI136756 · NIAID · UNIVERSITY OF MARYLAND BALTIMORE · PI LI, QINGSHENG, LI, YUXING · 2019 to 2023
$3.9M
Graduate Training in VirologyT32AI125186 · NIAID · UNIV OF MARYLAND, COLLEGE PARK · PI SIMON, ANNE ELIZABETH · 2016 to 2020
$1.3M
NIAID NIH HHS P01 AI104722NIAID NIH HHS P30 AI036214NIAID NIH HHS R01 AI102766NIAID NIH HHS R01 AI136756NIAID NIH HHS T32 AI125186NIAID NIH HHS UM1 AI100663
6 · The paper itself

Abstract

Here, we have established an antigen-specific single B cell sorting and monoclonal antibody (mAb) cloning platform for analyzing immunization- or viral infection-elicited antibody response at the clonal level in guinea pigs. We stained the peripheral blood mononuclear cells (PBMCs) from a guinea pig immunized with HIV-1 envelope glycoprotein trimer mimic (BG505 SOSIP), using anti-guinea pig IgG and IgM fluorochrome conjugates, along with fluorochrome-conjugated BG505 SOSIP trimer as antigen (Ag) probe to sort for Ag-specific IgG

Indexed as

B cellsenvelope glycoproteinsguinea pigHIV-1immunizationmonoclonal antibodiessingle cell RT-PCR

Identifiers

PMID31065249
PMCPMC6489837
OpenAlexW2942308596

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.