ArticleRNA (New York, N.Y.)2019
Single molecule chromogenic in situ hybridization assay for RNA visualization in fixed cells and tissues.
Article in RNA (New York, N.Y.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed, 13 citations in OpenAlex.
- Visualization of Individual RNA Molecules by Proximity Ligation-Based Chromogenic In Situ Hybridization Assay.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Deciphering a critical role of uterine epithelial SHP2 in parturition initiation at single cell resolution.Nature communications · 2023Article
- Single-molecule RNA in situ detection in clinical FFPE tissue sections by vsmCISH.RNA (New York, N.Y.) · 2023Article
- SCRINSHOT enables spatial mapping of cell states in tissue sections with single-cell resolution.PLoS biology · 2020Article
- Detection of Individual RNA in Fixed Cells and Tissues by Chromogenic ISH.Bio-protocol · 2020Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Visualization of gene expression at single RNA molecular level represents a great challenge to both imaging technologies and molecular engineering. Here we show a single molecule chromogenic in situ hybridization (smCISH) assay that enables counting and localizing individual RNA molecules in fixed cells and tissue under bright-field microscopy. Our method is based on in situ padlock probe assays directly using RNA as a ligation template and rolling circle amplification combined with enzyme catalyzed chromogenic reaction for amplification product visualization. We show potential applications of our method by detecting gene expression variations in single cells, subcellular localization information of expressed genes, and gene expression heterogeneity in formalin-fixed, paraffin-embedded tissue sections. This facile and straightforward method can in principle be applied to any type of RNA molecules in different samples.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.