ArticleEuro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin2019
Molecular identification of the source of an uncommon tularaemia outbreak, Germany, autumn 2016.
Article in Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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7 citing papers in PubMed.
- Article
- Tularemia Presenting Solely with Cervical Lymphadenopathy and Fever.Diagnostics (Basel, Switzerland) · 2022Article
- Ulceroglandular form of tularemia after squirrel bite: a case report.Journal of medical case reports · 2022Article
- Genotyping ofMicroorganisms · 2020Article
- Outbreak of Tularemia in a Group of Hunters in Germany in 2018-Kinetics of Antibody and Cytokine Responses.Microorganisms · 2020Article
- Review
- Genetic Diversity and Spatial Segregation ofFrontiers in cellular and infection microbiology · 2019Article
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7 authors.
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Abstract
BackgroundIn 2016, an uncommon outbreak of oropharyngeal tularaemia involving six human cases occurred in Germany, caused by drinking contaminated fresh must after a grape harvest.AimWe describe the details of laboratory investigations leading to identification of the outbreak strain, its characterisation by next generation sequencing (NGS) and the finding of the possible source of contamination.MethodsWe incubated wine samples in different media and on agar plates. NGS was performed on DNA isolated from young wine, sweet reserve and an outbreak case's lymph node. A draft genome of the outbreak strain was generated. Vertebrate-specific PCRs using primers targeting the mitochondrial cytochrome b gene and product analyses by blast search were used to identify the putative source of must contamination.ResultsNo bacterial isolate could be obtained. Analysis of the draft genome sequence obtained from the sweet reserve attributed this sequence to
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