ArticleBiotechnology and bioengineering2019
Product solubility control in cellooligosaccharide production by coupled cellobiose and cellodextrin phosphorylase.
Article in Biotechnology and bioengineering, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Reporter Group-Labeled Synthetic Cellulose: Structural Characterization and Utilization in Mapping the Cellulose Chain-Cleavage Modes of Cellulases.Biomacromolecules · 2026Article
- Spore immobilized enzymes for the multi-step synthesis of cellobiose.Microbial cell factories · 2026Article
- Production of cello-oligosaccharides from corncob residue by degradation-synthesis reactions.Applied microbiology and biotechnology · 2024Article
- Insights to improve the activity of glycosyl phosphorylases from RuminococcusFrontiers in chemistry · 2023Article
- Continuous process technology for bottom-up synthesis of soluble cello-oligosaccharides by immobilized cells co-expressing three saccharide phosphorylases.Microbial cell factories · 2022Article
- Inorganic phosphate self-sufficient whole-cell biocatalysts containing two co-expressed phosphorylases facilitate cellobiose production.Journal of industrial microbiology & biotechnology · 2022Article
- Engineering cascade biocatalysis in whole cells for bottom-up synthesis of cello-oligosaccharides: flux control over three enzymatic steps enables soluble production.Microbial cell factories · 2022Article
- Engineering analysis of multienzyme cascade reactions for 3'-sialyllactose synthesis.Biotechnology and bioengineering · 2021Article
- Recent advances in enzymatic synthesis of β-glucan and cellulose.Carbohydrate research · 2021Review
- A promiscuous glycosyltransferase generates poly-β-1,4-glucan derivatives that facilitate mass spectrometry-based detection of cellulolytic enzymes.Organic & biomolecular chemistry · 2021Article
- Kinetic modeling of phosphorylase-catalyzed iterative β-1,4-glycosylation for degree of polymerization-controlled synthesis of soluble cello-oligosaccharides.Biotechnology for biofuels · 2021Article
- β-Glucan phosphorylases in carbohydrate synthesis.Applied microbiology and biotechnology · 2021Review
- Engineering of cellobiose phosphorylase for the defined synthesis of cellotriose.Applied microbiology and biotechnology · 2020Article
- On the donor substrate dependence of group-transfer reactions by hydrolytic enzymes: Insight from kinetic analysis of sucrose phosphorylase-catalyzed transglycosylation.Biotechnology and bioengineering · 2020Article
- Short-Chain Cello-oligosaccharides: Intensification and Scale-up of Their Enzymatic Production and Selective Growth Promotion among Probiotic Bacteria.Journal of agricultural and food chemistry · 2020Article
- Product solubility control in cellooligosaccharide production by coupled cellobiose and cellodextrin phosphorylase.Biotechnology and bioengineering · 2019Article
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3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Soluble cellodextrins (linear β-1,4-d-gluco-oligosaccharides) have interesting applications as ingredients for human and animal nutrition. Their bottom-up synthesis from glucose is promising for bulk production, but to ensure a completely water-soluble product via degree of polymerization (DP) control (DP ≤ 6) is challenging. Here, we show biocatalytic production of cellodextrins with DP centered at 3 to 6 (~96 wt.% of total product) using coupled cellobiose and cellodextrin phosphorylase. The cascade reaction, wherein glucose was elongated sequentially from α-d-glucose 1-phosphate (αGlc1-P), required optimization and control at two main points. First, kinetic and thermodynamic restrictions upon αGlc1-P utilization (200 mM; 45°C, pH 7.0) were effectively overcome (53% → ≥90% conversion after 10 hrs of reaction) by in situ removal of the phosphate released via precipitation with Mg
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