Evidence map›Paper›PMID 31062353›Full record

ArticleJournal of anatomy2019

Surface contrast enhancement of integumentary structures in X-ray tomography.

Peter T Rühr, Markus Lambertz

Open access · hybridAbstract readEvaluation Study
In one paragraph

Article in Journal of anatomy, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 6 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 1 country.

Peter T RührInstitut für Zoologie, Universität zu Köln, Cologne, Germany.ORCID 0000-0003-2776-6172
Markus LambertzInstitut für Zoologie, Rheinische Friedrich-Wilhelms-Universität Bonn, Bonn, Germany.ORCID 0000-0001-8348-9347
University of Bonn · DEUniversity of Cologne · DE

Funding

H2020 European Research Council 754290
6 · The paper itself

Abstract

Micro-computed tomography (μCT) has become standard in the biological sciences to reconstruct, display and analyse 3D models of all kinds of organisms. However, it is often impossible to capture fine details of the surface and the internal anatomy at the same time with sufficient contrast. Here we introduce a new approach for the selective contrast-enhancement of integumentary surface structures. The method relies on conventional and readily available sputter coaters to cover the entire sample with a thin layer of gold atoms. This approach proved successful on a diverse array of plants and animals. On average, we achieved a 14.48-fold gain of surface contrast (ranging from 2.42-fold to 86.93-fold) compared with untreated specimens. Even X-ray-transparent samples such as spider silk became accessible via μCT. This selective contrast-enhancement, makes it possible to digitally reconstruct fine surface structures with low absorbance while the tissue-dependent grey value resolution of the inner anatomy is maintained and remains fully visualisable. The methodology is suited for a broad scientific application across biology and other sciences employing (μ)CT, as well as educative and public outreach purposes.

Indexed as

GoldAnimalsInsectaIntegumentary SystemSeedsX-Ray MicrotomographyGold3D morphologyreconstructionsputteringstainingvolume renderμCT

Identifiers

PMID31062353
PMCPMC6637458
OpenAlexW2944291820

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.