ArticleFEMS microbiology letters2019
Aspergillus niger citrate exporter revealed by comparison of two alternative citrate producing conditions.
Article in FEMS microbiology letters, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 34 citations in OpenAlex.
- The global regulator LaeA controls expression of genes involved in diverse primary and secondary metabolites biosynthesis and their secretion in fungi.Frontiers in microbiology · 2026Review
- Model-Driven Engineering of Yarrowia lipolytica for Improved Microbial Oil Production.Microbial biotechnology · 2025Article
- Identification ofJournal of fungi (Basel, Switzerland) · 2023Article
- Manganese(II) ions suppress the transcription of the citrate exporter encoding geneFrontiers in bioengineering and biotechnology · 2023Article
- Structural characterization of theComputational and structural biotechnology journal · 2023Article
- Phylogenomics of aiScience · 2022Article
- Uncovering Novel Plasma Membrane Carboxylate Transporters in the YeastJournal of fungi (Basel, Switzerland) · 2022Article
- Identification and genetic characterization of mitochondrial citrate transporters inFrontiers in microbiology · 2022Article
- Fungal Mobilization of Selenium in the Presence of Hausmannite and Ferric Oxyhydroxides.Journal of fungi (Basel, Switzerland) · 2021Article
- Making Traditional Japanese Distilled Liquor, Shochu and Awamori, and the Contribution of White and BlackJournal of fungi (Basel, Switzerland) · 2021Review
- The Role of Metal Ions in Fungal Organic Acid Accumulation.Microorganisms · 2021Review
- Global Transcriptional Response ofJournal of fungi (Basel, Switzerland) · 2021Article
- Overflow Metabolism inFrontiers in fungal biology · 2021Article
- Membrane transporters in the bioproduction of organic acids: state of the art and future perspectives for industrial applications.FEMS microbiology letters · 2020Review
- LaeA Controls Citric Acid Production through Regulation of the Citrate Exporter-EncodingApplied and environmental microbiology · 2020Article
- Aspergillus niger citrate exporter revealed by comparison of two alternative citrate producing conditions.FEMS microbiology letters · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Currently, there is no consensus regarding the mechanism underlying Aspergillus niger citrate biosynthesis and secretion. We hypothesise that depending on the experimental setup, extracellular citrate accumulation can have fundamentally different underlying transcriptomic landscapes. We show that varying the amount and type of supplement of an arginine auxotrophic A. niger strain results in transcriptional down-regulation of citrate metabolising enzymes in the condition in which more citrate is accumulated extracellularly. This contrasts with the transcriptional adaptations when increased citrate production is triggered by iron limitation. By combining gene expression data obtained from these two very distinct experimental setups with hidden Markov models and transporter homology approaches, we were able to compile a shortlist of the most likely citrate transporter candidates. Two candidates (An17g01710 and An09g06720m.01) were heterologously expressed in the yeast Saccharomyces cerevisiae, and one of the resultant mutants showed the ability to secrete citrate. Our findings provide steps in untangling the complex interplay of different mechanisms underlying A. niger citrate accumulation, and we demonstrate how a comparative transcriptomics approach complemented with further bioinformatics analyses can be used to pinpoint a fungal citrate exporter.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.