ArticleBiomedical optics express2019
Imaging the brain in 3D using a combination of CUBIC and immunofluorescence staining.
Article in Biomedical optics express, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Human cerebral organoids model tumor initiation and infiltration in an autologous astrocyte-supported setting.iScience · 2025Article
- 3D visualization of human colon tissue using a modified CUBIC-based tissue-clearing technique.Journal of biological methods · 2025Article
- Whole-brain 3D imaging of dopaminergic neurons and glial cells in the mouse model of Parkinson's disease induced by 6-OHDA.Frontiers in aging neuroscience · 2025Article
- Patients' Stem Cells Differentiation in a 3D Environment as a Promising Experimental Tool for the Study of Amyotrophic Lateral Sclerosis.International journal of molecular sciences · 2022Article
- Techniques for visualizing fibroblast-vessel interactions in the developing and adult CNS.Neurophotonics · 2022Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Three-dimensional (3D) high-resolution imaging can help us better understand the brain, and CUBIC is a powerful technique to render the brain transparent, thus benefiting 3D imaging. In this study, we combined CUBIC with immunofluorescence staining (IFS) and successfully acquired 3D high-resolution images of cerebral vessels, astrocytes, dopaminergic neurons and pericytes, providing us abundant information about the change in spatial frequency content for cellular scale features. Additionally, we found that a longer duration of antibody incubation produced deeper staining. Our study indicates the broad application prospects of IFS-based CUBIC for studying specific cerebral structures and diseases related to alterations of these structures.
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Registered trials
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