ArticleScientific reports2019
TickPath Layerplex: adaptation of a real-time PCR methodology for the simultaneous detection and molecular surveillance of tick-borne pathogens.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Hard Ticks as Vectors: The Emerging Threat of Tick-Borne Diseases in India.Pathogens (Basel, Switzerland) · 2024Review
- Development of a Syndromic Molecular Diagnostic Assay for Tick-Borne Pathogens Using Barcoded Magnetic Bead Technology.Microbiology spectrum · 2023Article
- Under-diagnosis of vector-borne diseases among individuals suspected of having Scrub Typhus in South Korea.PloS one · 2023Article
- Current and Future Molecular Diagnostics of Tick-Borne Diseases in Cattle.Veterinary sciences · 2022Review
- Capture Sequencing Enables Sensitive Detection of Tick-Borne Agents in Human Blood.Frontiers in microbiology · 2022Article
- Gap Analysis of the Habitat Interface of Ticks and Wildlife in Mexico.Pathogens (Basel, Switzerland) · 2021Review
- Challenges in Tick-Borne Pathogen Detection: The Case forPathogens (Basel, Switzerland) · 2021Review
- Microbiome Composition andTropical medicine and infectious disease · 2020Article
- Prevalence of common tick-borne pathogens in white-tailed deer and coyotes in south Texas.International journal for parasitology. Parasites and wildlife · 2020Article
- Prevalence of protozoan parasites in small and medium mammals in Texas, USA.International journal for parasitology. Parasites and wildlife · 2020Article
- ApparentJFMS open reportsArticle
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4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tick-borne diseases (TBD) are common across the United States and can result in critical and chronic diseases in a variety of veterinary patients. Moreover, borreliosis, anaplasmosis, rickettsiosis, ehrlichiosis, and babesiosis are zoonotic and have been cited as the most common TBDs. Molecular diagnostic methodologies utilized for screening domestic dogs for these causative agents include real-time PCR (qPCR) assays in both singleplex and multiplex formats. However, current limitations of qPCR instruments restrict the number of fluorogenic labels that can be differentiated by the instrument for a given reaction. This study describes the development of the TickPath Layerplex, a diagnostic assay based on qPCR methodology that was adapted for the simultaneous detection and characterization of 11 pathogens responsible for causing 5 common TBDs in domestic dogs. The analytical and diagnostic performance of the layerplex assay was evaluated and shown to be compatible with common instruments utilized in molecular diagnostic laboratories. Test results revealed no inhibition or reduction in sensitivity during validation of the layerplex assay, and the limit of detection was determined to be near 16 genome copy equivalents per microliter. Overall, the high sensitivity, specificity, and screening capability of the assay demonstrate its utility for broadly screening dogs for common TBDs.
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